华东师范大学学报(自然科学版) ›› 2009, Vol. 2009 ›› Issue (5): 94-99.

• 环境工程 生命科学 • 上一篇    下一篇

喜树内生真菌的分离及产10-羟基喜树碱菌的鉴定

闵长莉1， 袁 萍2   

  1. 1. 皖西学院 化学与生命科学系，安徽 六安 237012； 2. 上海师范大学 生命与环境科学学院，上海 200234
  • 收稿日期:2008-12-19 修回日期:2009-03-04 出版日期:2009-09-25 发布日期:2014-10-13
  • 通讯作者: 闵长莉

Isolation and identification of a 10-hydroxycamptothecin-producingendophytic fungus from Camptotheca acuminate Decne (Chinese)

MIN Chang-li1, YUAN Ping2   

  1. 1. Department of Chemistry and Life Science, West Anhui University, Liuan Anhui 237012, China; 2. Department of Biology and Environment Science, Shanghai Normal University,Shanghai 200234, China
  • Received:2008-12-19 Revised:2009-03-04 Online:2009-09-25 Published:2014-10-13
  • Contact: MIN Chang-li

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摘要： 从喜树（Camptotheca acuminate Decne）树皮中分离、纯化得到6株内生真菌，经摇瓶培养后，采用TLC法与HPLC法对其菌丝体提取物进行分析，结果表明其中1株内生真菌(XK002)产10-羟基喜树碱（10-hydroxycamptothecin,HCPT），将此菌株命名为XK002，其10-羟基喜树碱产量为410 μg/L.对菌株XK002菌落、菌丝体和孢子的有无进行了研究，表明菌株XK002属于无孢菌群(Mycelia sterlia).

关键词: 喜树内生真菌, 分离, 鉴定, 10-羟基喜树碱, 喜树内生真菌, 分离, 鉴定, 10-羟基喜树碱

Abstract: Six endophytic fungi isolated from Camptotheca acuminate Decne were cultured in taper flask under shaking. The organic extracts of mycelium were analyzed with thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). The results showed one of the strains produced the 10-hydroxycamptothecin (HCPT) with output of 410 μg/L and was named XK002. Morphological observation indicates that XK002 belongs to Mycelia sterlia.

Key words: isolation, identification, 10-hydroxycamptothecin, endophytic fungi of Camptotheca acuminate Decne, isolation, identification, 10-hydroxycamptothecin

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