城市河道介体强化总氮削减的微生物群落结构分析

doi:10.3969/j.issn.1000-5641.2018.06.012

华东师范大学学报(自然科学版) ›› 2018, Vol. 2018 ›› Issue (6): 97-104.doi: 10.3969/j.issn.1000-5641.2018.06.012

城市河道介体强化总氮削减的微生物群落结构分析

韩静^1,2, 许怡雯^1,2, 何岩^1,2, 黄民生^1,2

1. 华东师范大学生态与环境科学学院, 上海 200241;
2. 华东师范大学上海市城市化生态过程与生态恢复重点实验室, 上海 200241

收稿日期:2018-07-16 出版日期:2018-11-25 发布日期:2018-12-01
通讯作者: 何岩,女,副教授,硕士生导师,研究方向为水环境治理与修复.E-mail:yhe@des.ecnu.edu.cn. E-mail:yhe@des.ecnu.edu.cn
作者简介:韩静,女,硕士研究生,研究方向为水环境治理与修复.E-mail:jhanecnu@sina.com.
基金资助:
国家自然科学基金（41877477）；上海市自然科学基金（16ZR1408800）；上海市科技创新重点项目（18DZ1203806）；上海市浦江人才计划（16PJD023）

Analysis of microbial community composition from media used to strengthen total nitrogen removal from an urban river

HAN Jing^1,2, XU Yi-wen^1,2, HE Yan^1,2, HUANG Min-sheng^1,2

1. School of Ecological and Environmental Sciences, East China Normal University, Shanghai 200241, China;
2. Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, East China Normal University, Shanghai 200241, China

Received:2018-07-16 Online:2018-11-25 Published:2018-12-01

摘要/Abstract

摘要： 通过比较3种介体材料（生物炭、毛毡和无纺布）对城市河道总氮削减强化的效果，发现经过84 d的运行，相比空白组，无纺布对总氮的削减强化效果最好，削减率可提升23.4%；其次是毛毡，削减率可提升13.9%；生物炭对总氮削减效果不佳.在此基础上，系统探究了不同介体强化组中的微生物多样性及结构分布特征，表明与空白组相比，无纺布组可以显著增加菌群多样性和丰富度，而且与氮转化相关的变形菌门丰度明显高于毛毡组和生物炭组，特别是一些典型的反硝化菌属Azospirillum、Thiobacillus和Azoarcus的丰度均显著高于其他实验组，而与反硝化菌存在竞争关系的产甲烷菌属Syntrophorhabdus低于空白和生物炭组；毛毡组的微生物菌群结构呈现出与无纺布相似的趋势，但其中的反硝化菌属丰度均低于无纺布组；生物炭组微生物群落组成与空白组最为相似，推测无纺布介体材料具有较好的生物相容性，更有利于挂膜，从而促使反硝化菌属的生长.综合比较，无纺布介体材料更有利于对城市河道总氮的削减.

关键词: 介体材料, 城市河道, 总氮削减, 微生物作用

Abstract: We compared the removal rate of total nitrogen in urban rivers with three kinds of media (biochar, felt, and non-woven fabric). After 84 days in operation, it was found that non-woven fabric increased the removal rate of total nitrogen by 23.4% over the control group; felt increased the removal rate of total nitrogen by 13.9% over the control group; and biochar had a poor effect on total nitrogen removal. The microbial diversity and community composition of different media-enhanced groups were then systematically explored; the results showed that non-woven fabric can significantly increase microbial diversity and abundance. Moreover, proteobacteria, which is related to nitrogen conversion, was found to be significantly higher in the non-woven fabric group than the felt and biochar groups, especially the abundance of denitrifying bacteria (Azospirillum, Thiobacillus, and Azoarcus). The abundance of methanogen, which is in competition with the denitrifying bacteria Syntrophorhabdus, was found to be lower in non-woven fabric than in the control and biochar groups. The microbial flora structure of the felt group showed a similar trend to that of the non-woven fabric group, but the abundance of denitrifying bacteria was lower. The microbial community composition of the biochar group was the most similar to that of the control group. It is speculated that non-woven fabric has better biocompatibility than other groups; this is beneficial for biofilm formation and thereby promotes the growth of denitrifying bacteria. In a comprehensive comparison, non-woven media materials are more conducive to the removal of total nitrogen in urban rivers.

Key words: media, urban river, total nitrogen reduction, microbial action

中图分类号:

X522

韩静, 许怡雯, 何岩, 黄民生. 城市河道介体强化总氮削减的微生物群落结构分析[J]. 华东师范大学学报(自然科学版), 2018, 2018(6): 97-104.

HAN Jing, XU Yi-wen, HE Yan, HUANG Min-sheng. Analysis of microbial community composition from media used to strengthen total nitrogen removal from an urban river[J]. Journal of East China Normal University(Natural Sc, 2018, 2018(6): 97-104.

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城市河道介体强化总氮削减的微生物群落结构分析

Analysis of microbial community composition from media used to strengthen total nitrogen removal from an urban river

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