城市河道沉积物溶解性有机质特征及其对反硝化过程的影响研究

doi:10.3969/j.issn.1000-5641.2021.04.006

华东师范大学学报（自然科学版） ›› 2021, Vol. 2021 ›› Issue (4): 46-54.doi: 10.3969/j.issn.1000-5641.2021.04.006

城市河道沉积物溶解性有机质特征及其对反硝化过程的影响研究

翁蕊^1,^2,^3,⁴, 韦政^1,^2,^3,⁴, 杨燕梅^1,^2,^3,⁴, 韩静^1,^2,^3,⁴, 何岩^1,^2,^3,^4,*(), 黄民生^1,^2,^3,⁴

1. 华东师范大学生态与环境科学学院上海市城市化生态过程与生态恢复重点实验室, 上海　200241
2. 崇明生态研究院, 上海　202162
3. 上海有机固废生物转化工程技术研究中心, 上海　200241
4. 自然资源部大都市区国土空间生态修复工程技术创新中心, 上海　200062

收稿日期:2020-11-16 出版日期:2021-07-25 发布日期:2021-07-23
通讯作者: 何岩 E-mail:yhe@des.ecnu.edu.cn
基金资助:
国家自然科学基金 (41877477); 国家重大科技项目 (2018ZX07208008, 2017ZX07207001); 上海市科技创新重点项目 (18DZ1203806)

Characteristics of dissolved organic matter and its effects on denitrification in urban river sediments

Rui WENG^1,^2,^3,⁴, Zheng WEI^1,^2,^3,⁴, Yanmei YANG^1,^2,^3,⁴, Jing HAN^1,^2,^3,⁴, Yan HE^1,^2,^3,^4,*(), Minsheng HUANG^1,^2,^3,⁴

1. Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, School of Ecological and Environmental Sciences, East China Normal University, Shanghai　200241, China
2. Institute of Eco-Chongming, Shanghai　202162, China
3. Shanghai Engineering Research Center of Biotransformation of Organic Solid Waste, Shanghai　200241, China
4. Technology Innovation Center for Land Spatial Eco-Restoration in Metropolitan Area (Ministry of Natural Resources), Shanghai　200062, China

Received:2020-11-16 Online:2021-07-25 Published:2021-07-23
Contact: Yan HE E-mail:yhe@des.ecnu.edu.cn

摘要/Abstract

摘要：

为了解决城市河道治理过程中氮营养盐去除难题, 城市河道体系中溶解性有机质 (DOM) 对反硝化过程的影响作用值得重视. 研究表明, 河道沉积物中DOM的腐殖化程度较低、芳香性弱, 以小分子的富里酸为主, 其浓度平均为 (1868.5 ± 63.2) mg/kg. 与空白组相比, DOM可以促进反硝化过程, 对TN和NO₃^–-N去除率分别提升了7.24% ± 0.36%和23.52% ± 1.17%, 而DOM协同乙酸盐组对TN和NO₃^–-N的去除效果更好, 分别可以达到74.48% ± 1.29%和98.62% ± 0.07%. 微生物分析表明, DOM组的菌群多样性和丰富度均高于空白组, 但其中的异养反硝化菌属Pseudomonas和Brevundimonas以及nirK型反硝化菌属Paracoccus的丰度均低于DOM协同乙酸盐组. 此外, DOM运行体系中NH₄⁺-N浓度维持较高的水平 (大于3.7 mg/L), 而且含有DOM体系中与异化硝酸盐还原成铵 (DNRA) 功能相关的厌氧粘细菌 (Anaeromyxobacter), 其相对丰度明显增加, 推测DOM在促进反硝化的同时诱导了DNRA过程的发生.

关键词: 城市河道, 溶解性有机质, 反硝化, 富里酸, 异化硝酸盐还原成铵

Abstract:

Understanding the impact of dissolved organic matter (DOM) on the denitrification process is critical to addressing the challenges associated with nitrogen removal in urban river treatment. In this paper, we show that DOM in urban rivers are mainly comprised of small-molecule fulvic acids. The humic acid content and aromaticity of the DOM, moreover, were found to be low. Compared with the control case, DOM can promote the denitrification process; specifically, the removal efficiency of TN and NO₃^–-N in the DOM-added group increased by 7.24% ± 0.36% and 23.52% ± 1.17%, respectively. DOM with an acetate group had an even better effect on the removal of TN and NO₃^–-N, reaching 74.48% ± 1.29% and 98.62% ± 0.07%, respectively. Microbiological analysis showed that the DOM-added group can significantly increase the diversity and richness of the bacteria community compared with the control case. However, the relative abundance of the heterotrophic denitrifiers Pseudomonas and Brevundimonas as well as the nirK-type denitrifier Paracoccus in the DOM-added group was less than that of the DOM with an acetate group. Additionally, a relatively high concentration of NH₄⁺-N (> 3.7 mg/L) was observed in the DOM-added group. The addition of DOM can significantly increase the relative abundance of Anaeromyxobacter related to dissimilatory nitrate reduction to ammonium (DNRA) functional genes. It is speculated that DOM promotes the denitrification process and induces the DNRA process simultaneously.

Key words: urban river, dissolved organic matter, denitrification, fulvic acid, dissimilatory nitrate reduction to ammonium

中图分类号:

X522

翁蕊, 韦政, 杨燕梅, 韩静, 何岩, 黄民生. 城市河道沉积物溶解性有机质特征及其对反硝化过程的影响研究[J]. 华东师范大学学报（自然科学版）, 2021, 2021(4): 46-54.

Rui WENG, Zheng WEI, Yanmei YANG, Jing HAN, Yan HE, Minsheng HUANG. Characteristics of dissolved organic matter and its effects on denitrification in urban river sediments[J]. Journal of East China Normal University(Natural Science), 2021, 2021(4): 46-54.

图/表 7

表1

图1

图2

图3

表2

图4

图5

参考文献 31

1	何岩, 沈叔云, 黄民生, 等. 城市黑臭河道底泥内源氮硝化-反硝化作用研究. 生态环境学报, 2012, 21 (6): 1166- 1170.
2	王志霞, 葛小鹏, 晏晓敏, 等. 溶解性有机质对菲在沉积物上吸附与解吸性能的影响. 中国环境科学, 2012, 32 (1): 105- 112. doi: 10.3969/j.issn.1000-6923.2012.01.017
3	RIBOT M, COCHERO J, VAESSEN T N, et al. Leachates from helophyte leaf-litter enhance nitrogen removal from wastewater treatment plant effluents. Environmental Science & Technology, 2019, 53 (13): 7613- 7620.
4	HE Q C, FENG C P, CHEN N, et al. Characterizations of dissolved organic matter and bacterial community structures in rice washing drainage (RWD)-based synthetic groundwater denitrification. Chemosphere, 2019, 215, 142- 152. doi: 10.1016/j.chemosphere.2018.10.026
5	BAHAM J, SPOSITO G. Chemistry of water-soluble, metal-complexing ligands extracted from an anaerobically digested sewage sludge. Journal of Environmental Quality, 1983, 12 (1): 96- 100.
6	国家环保局. 水和废水监测分析方法 [M]. 4版. 北京: 中国环境科学出版社, 2002.
7	XU N, TAN G, WANG H, et al. Effect of biochar additions to soil on nitrogen leaching, microbial biomass and bacterial community structure. European Journal of Soil Biology, 2016, 74, 1- 8. doi: 10.1016/j.ejsobi.2016.02.004
8	PRIEME A, BRAKER G, TIEDJE J. Diversity of nitrite reductase (nirK and nirS) gene fragments in forested upland and wetland soils . Applied and Environmental Microbiology, 2002, 68 (4): 1893- 1900. doi: 10.1128/AEM.68.4.1893-1900.2002
9	ALLANA W, JOANNE C, SANFORD C, et al. Refined NrfA phylogeny improves PCR-based nrfA gene detection . Applied and Environmental Microbiology, 2014, 80 (7): 2110- 2119. doi: 10.1128/AEM.03443-13
10	傅平青, 刘丛强, 尹祚莹, 等. 腐殖酸三维荧光光谱特性研究. 地球化学, 2004, 33 (3): 301- 308. doi: 10.3321/j.issn:0379-1726.2004.03.010
11	WU F C, TANOUE E. Isolation and partial characterization of dissolved copper-complexing ligands in streamwaters. Environmental Science & Technology, 2001, 35, 3646- 3652.
12	傅平青, 刘丛强, 吴丰昌. 溶解有机质的三维荧光光谱特征研究. 光谱学与光谱分析, 2005, 25 (12): 2024- 2028. doi: 10.3321/j.issn:1000-0593.2005.12.031
13	黄量, 于德泉. 紫外光谱在有机化学中的应用 [M]. 北京: 科学出版社, 2000: 211-230.
14	闫彩虹. 湖泊沉积物溶解性有机质与有机氮特征研究 [D]. 长沙: 湖南农业大学, 2011.
15	周石磊, 张艺冉, 黄廷林, 等. 周村水库主库区水体热分层形成过程中沉积物间隙水DOM的光谱演变特征. 环境科学, 2018, 39 (12): 5451- 5463.
16	ARTINGER R, BUCKAU G, GEYER S, et al. Characterization of groundwater humic substances: influence of sedimentary organic carbon. Applied Geochemistry, 2000, 15 (1): 97- 116. doi: 10.1016/S0883-2927(99)00021-9
17	BLEYEN N, HENDRIX K, MOORS H, et al. Biodegradability of dissolved organic matter in Boom Clay pore water under nitrate-reducing conditions: Effect of additional C and P sources. Applied Geochemistry, 2018, 92, 45- 58. doi: 10.1016/j.apgeochem.2018.02.005
18	程琼, 庄婉娥, 杨丽阳. 水生系统中溶解态有机质的激发效应研究进展. 环境化学, 2018, 37 (1): 10- 18.
19	GAO L J, HAN F, ZHANG X W, et al. Simultaneous nitrate and dissolved organic matter removal from wastewater treatment plant effluent in a solid-phase denitrification biofilm reactor. Bioresource Technology, 2020, 314, 123714. doi: 10.1016/j.biortech.2020.123714
20	MIURA Y, WATANABE A Y, OKABE S. Significance of Chloroflexi in performance of submerged membrane bioreactors (MBR) treating municipal wastewater. Environmental Science & Technology, 2007, 41 (22): 7787- 7794.
21	HUG L A, CASTELLE C J, WRIGHTON K C, et al. Community genomic analyses constrain the distribution of metabolic traits across the Chloroflexi phylum and indicate roles in sediment carbon cycling. Microbiome, 2013, 1 (1): 22. doi: 10.1186/2049-2618-1-22
22	曹雁, 王桐屿, 秦玉洁, 等. 厌氧氨氧化反应器脱氮性能及细菌群落多样性分析. 环境科学, 2017, 38 (4): 1544- 1550.
23	李慧, 刘丹丹, 陈文清. 反硝化聚磷菌的筛选及脱氮除磷特性. 环境工程, 2016, 34 (4): 25- 28.
24	SHAO M, ZHANG T, FANG H H. Sulfur-driven autotrophic denitrification: diversity, biochemistry, and engineering applications. Applied Microbiology and Biotechnology, 2010, 88 (5): 1027- 1042. doi: 10.1007/s00253-010-2847-1
25	HAO R, LI S, LI J, et al. Denitrification of simulated municipal wastewater treatment plant effluent using a three-dimensional biofilm-electrode reactor: Operating performance and bacterial community. Bioresource Technology, 2013, 143, 178- 186. doi: 10.1016/j.biortech.2013.06.001
26	SHINODA Y, SAKAI Y, UENISHI H, et al. Aerobic and anaerobic toluene degradation by a newly isolated denitrifying bacterium, Thauera sp strain DNT-1. Applied and Environmental Microbiology, 2004, 70 (3): 1385- 1392. doi: 10.1128/AEM.70.3.1385-1392.2004
27	常玉梅, 杨琦, 郝春博, 等. 城市污水厂活性污泥强化自养反硝化菌研究. 环境科学, 2011, 32 (4): 1210- 1216.
28	聂毅磊, 贾纬, 曾艳兵, 等. 两株好氧反硝化聚磷菌的筛选、鉴定及水质净化研究. 生物技术通报, 2017, 33 (3): 116- 121.
29	武晓桐, 姜欣, 盛思远, 等. 静态好氧高温牛粪堆肥中nirK型反硝化细菌群落动态变化. 微生物学通报, 2019, 46 (5): 986- 996.
30	姚源, 竺建荣, 唐敏, 等. 好氧颗粒污泥技术处理乡镇污水应用. 环境科学研究, 2018, 31 (2): 379- 388.
31	卜翠娜. 异化硝酸盐还原菌 (DNRA) 的环境分布及富集培养研究 [D]. 济南: 山东大学, 2018.

名称	引物	序列	参考文献
细菌16S rRNA	338F	5′-ACTCCTACGGGAGGCAGCAG-3′	[7]
细菌16S rRNA	806R	5′-GGACTACNNGGGTATCTAAT-3′	[7]
反硝化nirK	nirK-876	5′-ATCATGGTSCTGCCGCG-3′	[8]
反硝化nirK	nirK-1040	5′-GCCTCGATCAGRTTGTGGTT-3′	[8]
异化硝酸盐还原nrfA	nrfA2aw	5′-CARTGYCAYGTBGARTA-3′	[9]
异化硝酸盐还原nrfA	nrfAR1	5′-TWNGGCATRTGRCARTC-3′	[9]

菌属	功能	菌属丰度/%			参考文献
菌属	功能	空白	DOM	DOM协同乙酸盐	参考文献
Thiobacillus (硫杆菌属)	硫自养反硝化	12.69	17.76	0.17	[23]
Pseudomonas (假单胞菌属)	异养反硝化	0.5	5.43	13.15	[24]
Thauera (陶厄氏菌属)	异养反硝化	8.8	0.8	0.06	[25-26]
Thermomonas (热单胞菌属)	硫自养反硝化	0.81	5.67	0.47	[27]
Brevundimonas (短波单胞菌属)	异养反硝化, 脱氮除磷	0.41	2.87	8.15	[28]

[1]	尹超, 李莹, 张婷月, 刘佳敏, 陈体达, 崔丹, 黄民生. 好氧反硝化菌的固定化及其效能研究[J]. 华东师范大学学报（自然科学版）, 2021, 2021(4): 1-7.
[2]	张红丽, 尹国宇, 郑艳玲, 高娟, 高灯州, 常永凯, 刘程. 沉积物再悬浮对长江口潮滩上覆水体脱氮过程的影响[J]. 华东师范大学学报（自然科学版）, 2020, 2020(3): 78-87.
[3]	汪珊, 朱瑾, 何岩, 黄民生, 周运昌. 硫化物对黑臭河道底泥反硝化潜势的影响作用研究[J]. 华东师范大学学报(自然科学版), 2019, 2019(4): 156-164.
[4]	韩静, 许怡雯, 何岩, 黄民生. 城市河道介体强化总氮削减的微生物群落结构分析[J]. 华东师范大学学报(自然科学版), 2018, 2018(6): 97-104.
[5]	何岩, 陈静涵, 黄民生, 许怡雯. 城市河道内源硫影响厌氧氨氧化过程的研究进展[J]. 华东师范大学学报(自然科学版), 2018, 2018(6): 68-73,130.
[6]	张婷月, 丁钰, 黄民生. 异养硝化-好氧反硝化细菌的筛选及其脱氮性能研究[J]. 华东师范大学学报(自然科学版), 2018, 2018(6): 22-31,87.
[7]	丁钰, 张婷月, 黄民生, 何岩, 曹承进. 好氧反硝化菌及其在污水处理和环境修复中的研究进展[J]. 华东师范大学学报(自然科学版), 2018, 2018(6): 1-11,67.
[8]	陈建军, 张建, 黄民生, 卢少勇. 硝酸盐介导底泥硫自养反硝化的过程、效应和应用[J]. 华东师范大学学报(自然科学版), 2015, 2015(2): 91-97.
[9]	祁佩时;林娜;刘云芝. 纺织纤维用于城市河道突发溢油污染的治理[J]. 华东师范大学学报(自然科学版), 2011, 2011(1): 156-162.
[10]	徐欢;张勇;黄民生;罗锦洪;何岩. 梯级生态浮床系统处理黑臭河水除磷性能研究[J]. 华东师范大学学报(自然科学版), 2011, 2011(1): 119-125.
[11]	刘成;胡湛波;郝晓明;柏燕艳. 城市河道黑臭评价模型研究进展[J]. 华东师范大学学报(自然科学版), 2011, 2011(1): 43-54.

城市河道沉积物溶解性有机质特征及其对反硝化过程的影响研究

Characteristics of dissolved organic matter and its effects on denitrification in urban river sediments

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 7

参考文献 31

相关文章 11

编辑推荐

Metrics

本文评价