Journal of East China Normal University(Natural Sc ›› 2004, Vol. 2004 ›› Issue (3): 93-98.

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The lmpact of Glutaraldehyde as Fixative on Marine Picophytoplankton

ZHANG Li-hua1, CHAO Min2   

  1. State Key Laboratory of Estuarine & Coastal Research, East China Normal University,Shanghai 200062,China
  • Received:2002-11-30 Revised:2003-01-14 Online:2004-09-25 Published:2004-09-25
  • Contact: ZHANG Li-hua

Abstract: Samples are fixed with 1% glutaraldehyde and stored in liquid Nitrogen .Cell density、size and intensity of autofluorescence are compared with fresh samples in this experiment . The results showed that cell density and cell size of all three picophytoplanktonic populations(prochlorococcus、 synechococcus and picoeukaryotes) increased, but intensity of autofluorescence decreased.

Key words: glutaraldehyde, picophytoplankton, flow cytometry, glutaraldehyde, picophytoplankton

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