Abstract: Using morphologic characters and internal transcribed spacers (ITS) sequence analysis and Inter-simple-sequence -repeat（ISSR）markers, 3 isolates wilt pathogens in potted plant of Chongming narcissus were detected. One was Nectria gliocladioides , while other two were Fusarium moniliforme var. subglutinans . Pathogenic test resulted in that N.gliocladioides was the advantage pathogen, and its optimum growth temperature is 25 ℃. The hypha couldn’t growth while the temperature is lower than 5 ℃ or higher than 40 ℃. The study would be helpful to prevent and cure blight of Chongming narcissus potted plant. ISSR markers are useful for distinguishing the pathogen stains. It could be a molecular assistant means for identification of fungal and species.

Key words: brown spot, ISSR, rDNA-ITS, narcissus ( Narcissus tazetta var. chinensis ), brown spot, ISSR, rDNA-ITS