A general decellularization method for preparing vascular scaffolds from blood vessels of different types, diameters and wall-thicknesses

Abstract

Abstract: This study was to build a general decellularization method by combining trypsinization with repeated frozen/thawing treatment for different types of vessels including saphenous vein, carotid artery and aorta. Saphenous vein, carotid artery and aorta were decellularized by trypsinization and repeated frozen/thawing treatment. The efficiencies of cell removal and extracellular matrix (ECM) integrity were examined by Hematoxylin and eosin staining, Masson Trichrome staining and Weigert’s staining. A quantitative means based on image analysis software was used to quantify the ECM preservation. Scanning electron microscopy was used to show the extracellular matrix integrity. Histology staining and quantitative analysis demonstrated trypsinization with repeated frozen/thawing treatment decellularized the saphenous vein, carotid artery and aorta completely. The ECM structure was optimally preserved and integrity. Scanning electron microscopy examination also showed the ECM was well-preserved and the fibers were dense and orderly. The present results revealed that the trypsinization with repeated frozen/thawing method is a promising one for preparing decellularized vascular scaffolds of different types, sizes and wall-thicknesses of blood vessels.

Key words: decellularization, extracellular matrix, saphenous vein, carotid artery, aorta

CLC Number:

ZHANG Hong-xia, ZHAI Wan-yin, ZHANG Hong-feng. A general decellularization method for preparing vascular scaffolds from blood vessels of different types, diameters and wall-thicknesses[J]. Journal of East China Normal University(Natural Sc, 2012, 2012(4): 50-60.

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