Abstract: In this study, a stable GPR41 receptor cell model was established. The GPR41 expression was detected by RT-PCR and western blot，while the function of GPR41 was confirmed by cAMP and Ca assays. These results have shown that we have successfully established GPR41 cell line which can be used for screening the agonists of the receptor in vitro. GPR41 receptor binding activity was tested by cAMP assay using secondary metabolites extracted from gulf seaweed aflatoxin c-f-3 in Putian Fujian. The results have also shown that the No.37 compound, a new compound belonging to 2-Pyrones, has GPR41 receptor agonist activity with high affinity. This is the first report on 2-Pyrones with GPR41 receptor agonist activity.

Key words: GPR41, stable cell line, secondary metabolites of marine fungi, agonist