Study on the effects of Mg2+ on the binding between Bloom syndrome helicase and G4DNA

Abstract

Abstract: This paper studied the effects of Mg²⁺ on G4DNA, BLM-G4DNA complex, and the binding of BLM642-1290 helicase and G4DNA using fluorescence anisotropy technology.The results indicated that the fluorescence anisotropy of G4DNA increased with Mg²⁺concentration (〖WTBX〗P〖WTBZ〗<0.01); The anisotropy of BLM-G4DNA complex showed varied tendency of decrease-increase-decrease with Mg²⁺ concentration (〖WTBX〗P〖WTBZ〗<0.01)；The anisotropy of the binding between the helicase and G4DNA gradually declined following Mg²⁺ concentration (〖WTBX〗P〖WTBZ〗<0.01); The binding was most likely to occur between the helicase and G4DNA when Mg²⁺ concentrations was 3.0 mmol/L, which suggested that the suitable amount of Mg²⁺ could promote the binding of the helicase and G4DNA but that could lead to the change of the binding properties of two molecules including the shape, flow ability, and charge. These results provided useful data for studying the interaction mechanism between BLM helicase and G4DNA.

Key words: BLM helicase, G4DNA, Mg²⁺, fluorescence polarization technology

CLC Number:

R733.7

LUO Heng, CAI Ming-juan, CHEN Xiang, DING Mei,LI Kun, XU Hou-qiang. Study on the effects of Mg²⁺ on the binding between Bloom syndrome helicase and G4DNA[J]. Journal of East China Normal University(Natural Sc, 20120, 2012(6): 81-88.

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Study on the effects of Mg²⁺ on the binding between Bloom syndrome helicase and G4DNA

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