Journal of East China Normal University(Natural Sc ›› 20120, Vol. 2012 ›› Issue (6): 122-130,138.doi:

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Species distribution and antibiotic susceptibility of Staphylococci isolated from the healthy human skins of East China Normal University students

JIANG De-ming, MA Hai-long, GULISITAN Nueraihemaiti,MAIERDAN Paliheti   

  1. School of Life Sciences, East China Normal University, Shanghai 200062, China
  • Received:2011-08-01 Revised:2011-11-01 Online:2012-11-25 Published:2013-01-11

Abstract: 〖WTBX〗Staphylococus〖WTBZ〗 were isolated from the healthy human skin samples using Baird Parker medium. The taxonomy and phylogenetic relationship of 〖WTBX〗Staphylococus 〖WTBZ〗were analyzed based on the 16S rRNA gene sequences. Antibiotic susceptibility of 〖WTBX〗Staphylococci〖WTBZ〗 was detected by B-K method. A total of 31 strains of 〖WTBX〗Staphylococus〖WTBZ〗 were isolated which can be classified into 6 different species. Each of 31 〖WTBX〗Staphylococus〖WTBZ〗 strains was able to resist to at least one antibiotic among 10 tested antibiotics. The highest resistance rate was found against Amoxicillin and Ampicillin with 91%. Only 3 strains among the isolated〖WTBX〗 Staphylococus〖WTBZ〗 strains were resistant to Amikacin. The results indicated that 〖WTBX〗Staphylococus〖WTBZ〗 strains were abundant in both Uyghur and Han’s skins and the population level of 〖WTBX〗Staphylococus〖WTBZ〗 species was higher in Uyghur human skin samples than in Han’s. Furthermore, our study also suggested that 〖WTBX〗Staphylococus〖WTBZ〗 strains isolated from healthy human skin samples of East China Normal University students had high resistance ability to 10 tested antibiotics.

Key words: skin microenvironment, 〖WTBX〗Staphylococci〖WTBZ〗, 16S rRNA gene, phylogenetic relationship, antibiotic susceptibility

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