Journal of East China Normal University(Natural Sc ›› 2013, Vol. 2013 ›› Issue (5): 61-70, 79.

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Crosslinking of decellularized bovine pericardium matrix by procyanidins: mechanical properties, stability and hemo-compatibility

MA Bing1,2, ZHAI Wan-yin2, ZHANG Jia-min3, SUN Xiao-ning4, ZHU Zi-yan3, ZHANG Hong-feng1, CHANG Jiang2   

  1. 1. School of Life Sciences, East China Normal University, Shanghai 200062, China;
    2. Biomaterials and Tissue Engineering Research Center, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China;
    3. Shanghai Blood Center, Shanghai Institute of Blood Transfusion, Shanghai 200051, China;
    4. Department of Cardiac Surgery, Zhongshan Hospital Fudan University, Shanghai 200032, China
  • Received:2012-07-01 Revised:2012-10-01 Online:2013-09-25 Published:2013-09-30

Abstract: In this study, decellularized bovine pericardium matrix (dbpECM) was cross-linked by using PC at series concentrations. Mechanical properties, stability and hemo-compatibility of PC-crosslinked dbpECM (PC-dbpECM) were investigated. The results show that the tensile strength of the PC-dbpECM which crosslinked by the 2.5 mg/mL PC is increased from 29.1 MPa to 51.3 MPa with the elastic modulus roughly at basic level. Resistance of PC-dbpECM to collagenase hydrolysis is equivalent to that of GA-crosslinked. When PC-dbpECM soaked in D-Hanks solution, only the excessive non-crosslinked PC released within a week, leaving the crosslinked PC stable all the experiment period. PC crosslinked dbpECM (1 mg/mL) can effectively inhibit calcification in vitro. Both PC- and GA-dbpECM have ideal hemo-compatibility, but the PC cross-linking is superior to that of GA. The rate of platelet adhesion on the surface of 2.5 mg/mL PC crosslinked dbpECM (7.8%) was significantly lower than that of non-crosslinked group (26.1%) and the GA crosslinked group (36.6%), indicating that PC-dbpECM has low coagulation spotential. These data suggest that PC-dbpECM has good mechanical properties, stability and hemo-compatibility, suitable for preparing biological heart valves.

Key words: procyanidins, decellularized bovine pericardium, mechanical properties, stability, hemo-compatibility

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