Journal of East China Normal University(Natural Sc ›› 2013, Vol. 2013 ›› Issue (6): 171-180.

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Analysis of gal-3 gene diversity of Bufo gargarizans

PAN Li-nian, WAN Wen-bin, ZHANG Shu-fang, ZHU-GE Hui, ZHOU Pin-pin, YANG Xian-yu   

  1. School of Forestry and Biotechnology, Zhejiang A&F University, Lin’an Zhejiang 311300, China
  • Received:2012-11-01 Revised:2013-03-01 Online:2013-11-25 Published:2014-01-13

Abstract: Three independent groups of first-strand cDNAs were synthesized from the total RNA extracted from the skin of a single individual of Bufo gargarizans using different kits including SMARTer TM RACE cDNA Amplification Kit and modified Quantscript RT kit, which were then used as templates for the subsequent polymerase chain reaction (PCR). The PCR products were cloned into the pGM-T vector and sequenced. As a result, 16 different uni-cDNAs were singled out of the total 31 cDNA clones. Same sequences, single nucleotide polymorphism sites (SNPs) and base deletions were detected in two or three independent experiments/groups, indicating gal-3 diversity, which led to diversity of proteins encoded. But alignment of the amino acid residues deduced form these gal-3 cDNAs revealed high conservation of glycosyl-binding sites, phosphorelation sites and the motifs highly associated with biological functions, which suggested the existence of galectin-3 diversity in the skin of B. gargarizans.

Key words: Bufo gargarizans, galectin-3, molecular diversity

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