Overexpression of URO gene repress the master switches of secondary cell wall development in Arabidopsis

Abstract

Abstract: There are several transcriptional factors that have been found taking part in secondary cell wall differentiation as master switches in Arabidopsis. However, how these master switches link to the major development event of plant is still unknown. Overexpression of URO (UP-RIGHT ROSETTE) gene leads to much higher free IAA concentration in uro mutant than that in wild type. In this work, the expression levels of the master switch genes were checked in uro/uro and uro/+ mutants through quantity PCR reactions. The results show that expressions of the master switch genes were significantly repressed while the expression of xylem development master switcher genes were not effected when URO gene was over expressing in uro/uro and uro/+ mutants. It can be concluded that URO gene takes part in the integration of secondary cell wall differentiation with the major development process of plant and URO gene could keep plant cell in a growth manner through inhibition of the secondary cell wall development.

Key words: secondary cell wall, auxin, master switches, URO(UP-RIGHT ROSETTE) gene

CLC Number:

Q945

NI Wei-ping, PANG Jing-yang, XU Yan-fei, LI Ling, LI Xiao-fang, SUN Yue. Overexpression of URO gene repress the master switches of secondary cell wall development in Arabidopsis[J]. Journal of East China Normal University(Natural Sc, 2014, 2014(3): 134-143.

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