Effects of PFOS exposure on lifespan in C. elegans and their relations with IIS genes

doi:10.3969/j.issn.1000-5641.2015.02.013

Abstract

Abstract: Using a model of C.elegans, this study aims to investigate the effect of PFOS on the lifespan and its mechanisms. Results showed that 0.2~200 μmol/L PFOS shortened the lifespan of wild type wormsin a concentrationdependent manner after 50 h exposure.In four transgenic strains, mutation of daf-16, daf-2 and age-1 which are related to Insulin/IGFl.1pathway(IIS), could affect the lifespans of nematodes. We further observed lifespan change rates of the four transgene C.elegansafter exposed to PFOS.Results showed that mutation of daf-16 or daf-2 did not alter PFOS induced effects of shortening lifespan in CF1139 and CF1580 strain.However, knockout of daf-16b or mutation of age-1blocked PFOS induced effects of shortening lifespan in CF1295 and TJ1052 strain. Our results suggested that PFOS could accelerate aging and shorten the lifespan of nematodes. PFOSinduced effects are closely relative with IIS signaling pathways, in which daf-16b and age-1 play important roles.

Key words: PFOS;C.elegans, lifespan, IIS signaling pathway, daf-16b, age-1

CLC Number:

X592

LI Dan, WU Jing-Xuan, LI Jia, HE De-Fu. Effects of PFOS exposure on lifespan in C. elegans and their relations with IIS genes[J]. Journal of East China Normal University(Natural Sc, 2015, 2015(2): 106-112.

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