An algorithm for precise image registration based on priori mark features

doi:10.3969/j.issn.1000-5641.2021.03.008

Abstract

Abstract:

The use of a gene sequencer requires that the lens and gene chip are aligned accurately before base-calling. We propose an algorithm to calculate the deviation of the field of view (FOV) from the ideal position. Marks are set at locations on the gene chip in advance, so that the deviation in position of the lens relative to the gene chip can be analyzed. Firstly, the marked locations are captured by extracting grayscale features of the image to initially align the center of the FOV; secondly, the coordinates for multiple key points on the marks are captured; and finally, the location and angle deviations are calculated by mapping coordinates for the key points. Practical and experimental analysis show that the image registration algorithm designed in this paper can achieve a high-precision estimate for the position deviation between the FOV and the gene chip.

Key words: image registration, position calibration, gene chip

CLC Number:

TP391.7

Tianbi LIU, Rui FENG. An algorithm for precise image registration based on priori mark features[J]. Journal of East China Normal University(Natural Science), 2021, 2021(3): 65-77.

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仪器分辨率/像素	cell大小/像素	亮度串扰/%	track宽度/像素	track数量/个		位置误差/像素	角度误差/(°)
仪器分辨率/像素	cell大小/像素	亮度串扰/%	track宽度/像素	横向	竖向	位置误差/像素	角度误差/(°)
2 560 × 2 160	9 × 9	15	9	8	8	0.243 1	0.010 882
2 560 × 2 160	6 × 6	10	6	8	8	0.274 6	0.012 292
5 012 × 5 012	9 × 9	20	9	9	9	0.284 9	0.006 376
5 012 × 5 012	6 × 6	15	6	9	9	0.330 5	0.007 397