Disinfection effect of seven frequently used disinfectants on bacteriophage MS2

doi:10.3969/j.issn.1000-5641.2023.02.017

Abstract

Abstract:

In this study, the MS2 phages were used as an indicator microorganism to test the function of seven disinfectants with different components: hydrogen peroxide, ethanol, fermented lactic acid disinfectant, iodine disinfectant, quaternary ammonium disinfectant, chlorine-containing disinfectant, and peracetic acid disinfectant. Our results showed that the virus disinfection rate varied notably between the selected disinfectants. The iodine disinfectant exhibited the strongest disinfection effect, followed by the quaternary ammonium salt disinfectant and the peracetic acid disinfectant, while the disinfection effects of hydrogen peroxide, ethanol, and fermentation lactic acid disinfectant were inadequate. The test results provide a reference for the efficient utilization of various disinfectants to eliminate harmful microorganisms in the environment.

Key words: disinfectant, MS2 phage, disinfection rates, disinfection logarithm values

CLC Number:

R187+.2

Yu LI, Zhenming GE. Disinfection effect of seven frequently used disinfectants on bacteriophage MS2[J]. Journal of East China Normal University(Natural Science), 2023, 2023(2): 161-167.

Figures/Tables 5

Fig.1

Table 1

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Table 2

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单位: ×10⁶ pfu/mL
组别	双氧水	乙醇	发酵乳酸消毒剂	络合碘消毒剂	复合季铵盐消毒剂	含氯消毒剂	过氧乙酸消毒剂
①消毒剂 $+ $ MS2噬菌体	14.0 $\pm $ 2.0	19.0 $\pm $ 4.6	62.0 $\pm $ 14.9	1.3 $\pm $ 0.6	26.0 $\pm $ 10.4		28.0 $\pm $ 19.3
②(消毒剂 $+ $ MS2噬菌体) $+ $ 中和剂	21.0 $\pm $ 3.6	20.0 $\pm $ 7.8	46.0 $\pm $ 7.9	1.4 $\pm $ 0.5	30.0 $\pm $ 11.1		93.0 $\pm $ 10.6
③中和剂 $+ $ MS2噬菌体	300.0 $\pm $ 91.7	68.0 $\pm $ 9.2	110.0 $\pm $ 18.0	68.0 $\pm $ 12.5	490.0 $\pm $ 134.5	68.0 $\pm $ 8.2	140.0 $\pm $ 39.4
④(中和剂 $+ $ 消毒剂) $+ $ MS2噬菌体	320.0 $\pm $ 70.0	49.0 $\pm $ 7.9	90.0 $\pm $ 12.1	56.0 $\pm $ 17.1	700.0 $\pm $ 192.1	84.0 $\pm $ 18.1	150.0 $\pm $ 56.1
⑤MS2噬菌体	280.0 $\pm $ 45.8	71.0 $\pm $ 22.6	120.0 $\pm $ 25.1	59.0 $\pm $ 7.9	650.0 $\pm $ 218.6	71.0 $\pm $ 12.5	140.0 $\pm $ 36.5
⑥中和剂阴性对照	0	0	0	0	0	0	0

指标	组别	平方和	自由度	均方	F值	p值
灭活率	组间	0.476	6	0.079	19.538	< 0.01
	组内	0.056	14	0.004
	总计	0.531	20
灭活对数值	组间	16.118	6	2.686	16.169	< 0.01
	组内	2.326	14	0.166
	总计	18.444	20