Journal of East China Normal University(Natural Science) >

2015 , Vol. 2015 >Issue 2: 84 - 90

DOI: https://doi.org/10.3969/j.issn.1000-5641.2015.02.010

Article

Responses of bacterial community structure in malodorous river sediments to aeration by DGGE

  • HE Yan ,
  • YAO Li-Ping ,
  • LI Wen-Chao ,
  • HUANG Min-Sheng ,
  • ZHANG Yi-Fan
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  • 1. Shanghai Key Lab for Urban Ecological Processes and EcoRestoration, East China Normal University, Shanghai200241, China; 
    2. School of Ecological and Environmental Sciences, East China Normal University, Shanghai200241, China

Received date: 2014-08-07

  Online published: 2015-05-28

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Abstract

In this study, 16S rDNA based PCR amplification and denaturing gradientgel electrophoresis (PCRDGGE) as well as sequence alignment from the excised DGGE bands were used to investigate the bacterial diversity and variation. In addition, the redundancy analysis was adopted to assess the relationship between bacterial community structure and environmental factors. The results showed that aeration had marked effects on bacterial community structure in malodorous river sediments and different aeration intensities resulted in variable bacterial community. Amongst them the highest richness of bacterial community was observed with the aeration turbulence of Re=1 810 and DO of 7.35. It was also inferred that moderate aeration facilitated the growths of related bacteria to N and Scycling and theProteobacteria was dominant. It was also found that DO and Re played important roles in bacterial community from river sediments.

Key words： malodorous river; sediments; aeration; bacteria; DGGE

Cite this article

HE Yan , YAO Li-Ping , LI Wen-Chao , HUANG Min-Sheng , ZHANG Yi-Fan . Responses of bacterial community structure in malodorous river sediments to aeration by DGGE[J]. Journal of East China Normal University(Natural Science), 2015 , 2015(2) : 84 -90 . DOI: 10.3969/j.issn.1000-5641.2015.02.010

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