Journal of East China Normal University(Natural Science) >

2015 , Vol. 2015 >Issue 2: 106 - 112

DOI: https://doi.org/10.3969/j.issn.1000-5641.2015.02.013

Article

Effects of PFOS exposure on lifespan in C. elegans and their relations with IIS genes

  • LI Dan ,
  • WU Jing-Xuan ,
  • LI Jia ,
  • HE De-Fu
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  • 1. School of Ecological and Environmental Sciences, East China Normal University, Shanghai200241, China;
    2. Shanghai Key Lab for Urban Ecological Processes and Eco Restoration, East China Normal University, Shanghai200241, China

Received date: 2014-09-12

  Online published: 2015-05-28

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Abstract

Using a model of C.elegans, this study aims to investigate the effect of PFOS on the lifespan and its mechanisms. Results showed that 0.2~200 μmol/L PFOS shortened the lifespan of wild type wormsin a concentrationdependent manner after 50 h exposure.In four transgenic strains, mutation of daf-16, daf-2 and age-1 which are related to Insulin/IGFl.1pathway(IIS), could affect the lifespans of nematodes. We further observed lifespan change rates of the four transgene C.elegansafter exposed to PFOS.Results showed that mutation of daf-16 or daf-2 did not alter PFOS induced effects of shortening lifespan in CF1139 and CF1580 strain.However, knockout of daf-16b or mutation of age-1blocked PFOS induced effects of shortening lifespan in CF1295 and TJ1052 strain. Our results suggested that PFOS could accelerate aging and shorten the lifespan of nematodes. PFOSinduced effects are closely relative with IIS signaling pathways, in which daf-16b and age-1 play important roles.

Key words： PFOS;C.elegans; lifespan; IIS signaling pathway; daf-16b; age-1

Cite this article

LI Dan , WU Jing-Xuan , LI Jia , HE De-Fu . Effects of PFOS exposure on lifespan in C. elegans and their relations with IIS genes[J]. Journal of East China Normal University(Natural Science), 2015 , 2015(2) : 106 -112 . DOI: 10.3969/j.issn.1000-5641.2015.02.013

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