Journal of East China Normal University(Natural Science) >

2016 , Vol. 2016 >Issue 3: 102 - 114

DOI: https://doi.org/2016.03.012

Article

Extracellular ATP affects the copper–induced cell death and H2O2 production in tobacco (Nicotiana tabacum L) cell-suspension cultures

  • BAI Jing-Yue ,
  • FENG Han-Qing ,
  • GUAN Dong-Dong ,
  • SUN Kun ,
  • JIA Ling-Yun ,
  • LI Qiao-Xia ,
  • ZHANG Jin-Ping
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Received date: 2015-06-19

  Online published: 2016-09-22

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Abstract

Extracellular ATP (eATP) has been considered as a mediating signal in several physiological processes of plants. In this article we showed that eATP can affect the copper-induced cell death and hydrogen peroxide (H_{2}O_{2}) production. CuCl_{2} at concentrations from 100 to 700 textit{mu }molL caused a
significant increase of cell death in tobacco (textit{Nicotiana tabacum} L) suspension cultures, and this increase of cell death level was followed with increases of both intracellular and extracellular H_{2}O_{2} production. The cells exposed to 300 textit{mu }molL CuCl_{2} were chosen to investigate the mechanisms for the copper-induced increases of cell death and H_{2}O_{2} production and the effect on this process. The results showed that the treatment_{ }with CuCl_{2 } at this concentration increased the activity of NADPH oxidase, and addition of DPI (diphenylene iodonium, an inhibitor of NADPH oxidase)alleviated the CuCl_{2}-induced increases of cell death and H_{2}O_{2} production, indicating that the CuCl_{2}-induced increases of cell death and H_{2}O_{2} production were related to an increase of the activity of NADPH oxidase. Addition of exogenous ATP at 50 textit{mu }molL into the CuCl_{2}-stressed cells further enhanced the levels of cell death,H_{2}O_{2} production, and NADPH oxidase activity. However, in the presence of DPI, exogenous ATP failed to do so. These observations indicated that eATP can affect the copper-induced changes of cell viability and H_{2}O_{2} production bystimulating NADPH oxidase

Key words： cell death;Cu^{2+ }ions;quad extracellular ATP; H_{2}O_{2;

Cite this article

BAI Jing-Yue , FENG Han-Qing , GUAN Dong-Dong , SUN Kun , JIA Ling-Yun , LI Qiao-Xia , ZHANG Jin-Ping . Extracellular ATP affects the copper–induced cell death and H2O2 production in tobacco (Nicotiana tabacum L) cell-suspension cultures[J]. Journal of East China Normal University(Natural Science), 2016 , 2016(3) : 102 -114 . DOI: 2016.03.012

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