The balance between supply and demand of ecosystem services is essential for sustainable development, while differentiated partitioning is important for optimal resource allocation. Based on the carnegie-ames-stanford-approach (CASA), the water balance equation, and the revised universal soil loss equation (RUSLE), this study depicts the supply and demand of carbon fixation and water and soil conservation services and their relationship in the Yangtze River Delta integration demonstration zone from 2000 to 2020. Self-organizing map (SOM) K-means two-order clustering technology is used to identify ecosystem service clusters dividing the ecological management zone. Management strategies are then proposed. The results show that: ① The supply of carbon fixation services continued to decrease but the demand increased. Additionally, the supply and demand of water and soil conservation services showed an increasing trend, and supply was less than demand. ② The supply-demand ratio of carbon fixation and soil conservation services showed an upward trend, whereas the supply-demand ratio of water conservation services showed a downward trend. Significant spatial differences were observed in the supply-demand ratio of ecosystem services. ③ Cluster analysis divided the demonstration area into different types of ecological regulation zones. The urban clusters in Wujiang and Shengze town districts are mainly to improve the capacity of water conservation services. The urban cluster in Qingpu District is to promote the water conservation and carbon fixation services capacity. The pilot start-up area and urban cluster in Jiashan City have a small gap between supply and demand, which focus on comprehensive protection. This study can provide decision-making support for resource allocation, ecological compensation, and coordinated development of regional integration in the demonstration area.