Journal of East China Normal University(Natural Sc ›› 2016, Vol. 2016 ›› Issue (2): 144-152.doi: 10.3969/j.issn.1000-5641.2016.02.016

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Ultrasensitive detection of protein biomarker using fluorescent quantum dot probes

 WU  Si-Min1,2, JIA  Chun-Ping2, LIU  Li-Fen2, GAO  Wan-Lei2, JING  Feng-Xiang2, Jin-Qing-Hui2, , ZHANG  Hong-Feng1, ZHAO  Jian-Long2   

  • Received:2015-03-23 Online:2016-03-25 Published:2016-07-25

Abstract: In this study, based on the unique properties of quantum dots (QDs) and immune magnetic beads, an fluorescent assay was built to detect low concentration carcinoembryonic antigen (CEA). The assay was carried out in a sandwich formation in which two antibodies simultaneously recognized two separate binding sites on the same target CEA. After enrichment with magnetic separator, the fluorescent intensities produced by the QDs, which were confined to the surface of the beads, could be read efficiently to quantify the concentration of the CEA. For this assay, the limit of detection was 38 pg/mL with the linear range was from 0.39 to 50 ng/mL, and it has been proved with high accuracy and repeatability in tumor marker control. With the high sensitivity, specificity and low sample consumption, this method takes a big step to the early diagnosis of diseases.

Key words: quantum dots, immunofluorescence assay, magnetic beads, tumor marker

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