Journal of East China Normal University(Natural Sc ›› 2009, Vol. 2009 ›› Issue (5): 94-99.

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Isolation and identification of a 10-hydroxycamptothecin-producingendophytic fungus from Camptotheca acuminate Decne (Chinese)

MIN Chang-li1, YUAN Ping2   

  1. 1. Department of Chemistry and Life Science, West Anhui University, Liuan Anhui 237012, China; 2. Department of Biology and Environment Science, Shanghai Normal University,Shanghai 200234, China
  • Received:2008-12-19 Revised:2009-03-04 Online:2009-09-25 Published:2014-10-13
  • Contact: MIN Chang-li

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Abstract: Six endophytic fungi isolated from Camptotheca acuminate Decne were cultured in taper flask under shaking. The organic extracts of mycelium were analyzed with thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). The results showed one of the strains produced the 10-hydroxycamptothecin (HCPT) with output of 410 μg/L and was named XK002. Morphological observation indicates that XK002 belongs to Mycelia sterlia.

Key words: isolation, identification, 10-hydroxycamptothecin, endophytic fungi of Camptotheca acuminate Decne, isolation, identification, 10-hydroxycamptothecin

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