Journal of East China Normal University(Natural Sc ›› 2012, Vol. 2012 ›› Issue (5): 54-61.

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Study on carbamate pesticides by capillary electrophoresis with laser-induced fluorescence detection

YANG Yu-lin 1, ZHAO Xiao-yin 2, ZHAO Wen-jie 2, ZHOU Tian-shu 2   

  1. 1. SGS-CSTC Standards Technical Service (Shanghai), Ltd., Shanghai 200233, China;
    2. Department of Environment Science, East China Normal University, Shanghai 200062, China
  • Received:2011-09-01 Revised:2011-12-01 Online:2012-09-25 Published:2012-09-29

Abstract: A sensitive and rapid capillary electrophoresis method with laser-induced fluorescence detection (CE-LIF) was developed for the determination of carbamate pesticides (including (3,5-dimethylphenyl) N-methylcarbamate (XMC), and mercaptodimethur). In alkaline solution, carbamate was hydrolyzed to methylamine, which can be derivatized by 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) and detected by CE-LIF. Several parameters which influence the hydrolysis, derivatization and electrophoresis separation, such as pH, concentration of buffer solution and concentration of NBD-Cl, were investigated. The detection limits of the three carbamates were 3.0×10-9、1.0×10-9 and 3.0×10-9 mol/L, respectively. The proposed method was successfully employed to determine the rate constants and activation energies for carbamates hydrolysis.

Key words: capillary electrophoresis, laser-induced fluorescence, carbamate pesticides, rate constant, activation energy

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