Journal of East China Normal University(Natural Sc ›› 2013, Vol. 2013 ›› Issue (5): 96-101.

• Article • Previous Articles     Next Articles

Investigation on chemiluminescence detection of vitamin B12 with capillary electrophoresis

ZHU Jin-kun1,2, SHU Lu1, WU Min1, WANG Qing-jiang1, HE Pin-gang1, FANG Yu-zhi1   

  1. 1. Department of Chemistry, East China Normal University, Shanghai 200241, China
    2.  College of Science, Anhui Science and Technology University,Fengyang Anhui 233100, China
  • Received:2012-06-01 Revised:2012-09-01 Online:2013-09-25 Published:2013-09-30

Abstract: Based on the chemiluminescence (CL) catalysis of vitamin
B12 (VB12 in luminol-H$_{2}$O$_{2}$ system, a novel
capillary electrophoresis-chemiluminescence (CE-CL) method was
developed for the accurate determination of this important nutrient
substance. First of all, sodium hydrosulfite
(Na$_{2}$S$_{2}$O$_{4})$ was found to be used as an competent
reduction reagent to pre-reduce VB12(Co(III)) into
VB12(Co(II)), which produced strong CL emission in the CL
reaction of luminol-H$_{2}$O$_{2}$. In this process, some
interfering CL noise was observed and capillary electrophoresis (CE)
was taken to remove it by efficient separation. In this way,
VB12 could be reduced gently and determined accurately by the
catalyzed CL signal with our lab-constructed CE-CL system. The
proposed CE-CL determination could be finished in 20 min with a
detection limit (LOD) of 2$\times $10$^{-7}$ mol/L ($S$/$N$=3), a
linear range from 6$\times $10$^{-7}$ to 6$\times$10$^{-4}$ mol/L
($r^{2}$=0.968) and a relative standard deviation (RSD) of 2.4{\%}
($n$=9). Utilizing this method, satisfactory results were obtained
by testing VB12 tablets with a recovery of 97{\%}$\sim
$106{\%}. Compared to previously reported CL analysis of VB12,
we dealt with the interference components in pre-reducing solution
for the first time to gain more reliable data with convenient

Key words: vitamin B12, chemiluminescence, capillary electrophoresis, sodium hydrosulfite

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