Journal of East China Normal University(Natural Sc ›› 2014, Vol. 2014 ›› Issue (4): 113-121, 179.

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Isolation and identification of lignin decomposing bacteria and cloning of lip gene in termite gut

GAO Yun-hang1,2,  WANG Wei1,  LIU Meng1, GOU Chang-long1,  LOU YU-jie1, MA Hong-xia1   

  1. 1. College of Animal Science and Technology,\ Jilin Agricultural University, Changchun} 130118, China; 2. Key Laboratory of Animal Production, Product Quality and Security, Jilin Agricultural University, Changchun 130118, China
  • Received:2013-10-01 Revised:2014-01-01 Online:2014-07-25 Published:2014-07-25

Abstract: A strain of bacterium that named PY12 was isolated from
the termite gut using Azure B method, which had an efficiently
lignin decomposition function. It was identificated as
\textit{Enterobacter hormaechei}. by morphological, biochemical
identification and\\ 16S rRNA identification. The $lip$ gene were
amplified by the specific primers, and then cloning it into pMD19-T
vector, the target fragment sequences were 918 bp, Homology
comparison with the $lip$ gene which known in GenBank, the Homology
was low, but when the nucleotide sequences were translated into
protein, the homology was up to 88.5{\%} with the Pseudomonas sp..

Key words: Termite, lignin decomposing bacteria, 16S rRNA, lignin peroxidase gene

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