Journal of East China Normal University(Natural Sc ›› 2005, Vol. 2005 ›› Issue (5/6): 183-187.

• Article • Previous Articles     Next Articles

Effects of the Hydrolysate of Nitrogen Sources on Transglutaminase by Streptomyces(Chinese)

CHANG Zhong-yi 1 ,YANG Xue-xia,ZHUANG Xiao-hui,HUANG Xiu-qin,GAO Hong-liang   

  1. 1. Schools of life science, East China Normal University, Shanghai200062, China;2. Colloge of Chemistry and Chemical Engieering,Donghua University,Shanghai200054,China
  • Received:2004-05-12 Revised:2004-09-15 Online:2005-12-31 Published:2005-12-31
  • Contact: GAO Hong-liang

Abstract: The effect of hydrochloric acid hydrolysis of soybean cake powder and cottonseed powder under different pressure were investigated on MTGase production by Streptomyces.The results showed that hydrolysate of soybean cake powder in high pressure served as favorable source.MTGase activity could reach 3.86 μmol/(min•mL).The optimum of hydrolysis of soybean cake powder is followed:suitable concentration of hydrochloric acid is 3 mol/L,hydrodysis time is 4 hours. The experimental results showed that the most suitable fermentation medium consisted of 1.0% peptone,1.5% hydrolysate of soybean cake powder.

Key words: acid hydrolysis, MTGase, soybean cake powder, acid hydrolysis, MTGase

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