Journal of East China Normal University(Natural Sc ›› 2006, Vol. 2006 ›› Issue (4): 16-22.

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Effect of Pulsed Electric Field on the Suspension Cultures of Taxus chinensis(Chinese)

HUANG Lin-ling1, YE Hong1, CHEN Shu-de1, ZHONG Jian-jiang2   

  1. 1.Key Laboratory of Optical and Magnetic Resonance Spectroscopy, Minsitry of Education, Department of Physics, East China Normal University,Shanghai 200062,China；  2.State Key Laboratory of Bioreactor Engineering, East ChinaUniversity of Science and Technology, Shanghai 200237, China
  • Received:2004-12-24 Revised:2005-04-16 Online:2006-07-25 Published:2006-07-25
  • Contact: CHEN Shu-de

Abstract: Two dimensional gel electrophoresis(2-DE) was used to analyse changes of the protein expression of suspension cultures of Taxus chinensis 48 hours after pulsed electric field (PEF) elicitation. The 2-DE experiments were repeated for 3 times, and proved to be reliable according to the correlation coefficient analysis. Distinct changes were found from comparison of the protein patterns of control and PEF treated cells. Matrixassisted laser desorption/ionization mass spectrometry and electrospray ionization mass spectrometry were used to analyse some proteins. The results indicated that heterogeneous nuclear ribonucleoprotein(hnRNP), aldose l-epimerase family protein and Ferredoxin-NADP(+) reductase, putative/adrenodoxin reductase are related to the induction of PEF.

Key words: Taxus chinensis, two dimensional gel electrophoresis, matrix-assisted laser desorption/ionization mass spectrometry, electrospray ionization mass spectrometry, pulsed electromagnetic field (PEF), Taxus chinensis, two dimensional gel electrophoresis, matrix-assisted laser desorption/ionization mass spectrometry, electrospray ionization mass spectrometry

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