Journal of East China Normal University(Natural Sc ›› 2005, Vol. 2005 ›› Issue (2): 91-97.
• Article • Previous Articles Next Articles
MO Bei-xin1,JIANG Li-wen2
Received:
Revised:
Online:
Published:
Contact:
Abstract: It is difficult for membrane proteins to get into 2-D gel because of their hydrophobic properties and the contaminations of carbohydrates in protein samples also affect the results of 2-D gel. The modified 2-D electrophoresis technique described in this paper overcomes these two difficulties, and have successfully separated proteins from sucrose gradient ultracentrifugation and got membrane proteins into 2-D gel. Proteomic analysis was then performed on proteins from prevacuolar compartment. Around 200 protein spots were separated on the 2-D gel, 23 protein spots were cut out form the gel and subjected to MS/MS analysis. After database searching, 13 proteins did not hit matching proteins, with the 10 proteins that hit matching proteins, 4 of them have known protein function, 6 of which turned out to be unknown or unnamed proteins.
Key words: peptide mass finger print, prevacuolar compartment, membrane protein, tobacco, 2-D electrophoresis, peptide mass finger print, prevacuolar compartment, membrane protein, tobacco
CLC Number:
Q946
MO Bei-xin;JIANG Li-wen. Identification of Proteins from Tobacco Prevacuolar Compartment by Two-Dimensional Electrophoresis and Peptide Mass Fingerprinting(Chinese)[J]. Journal of East China Normal University(Natural Sc, 2005, 2005(2): 91-97.
0 / / Recommend
Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks
URL: https://xblk.ecnu.edu.cn/EN/
https://xblk.ecnu.edu.cn/EN/Y2005/V2005/I2/91