Journal of East China Normal University(Natural Sc ›› 2009, Vol. 2009 ›› Issue (6): 134-140.

• Article • Previous Articles    

Cloning and expression pattern analysis of a cDNA of pkc-2gene in Caenorhabditis elegans (Chinese, Notes)

QIAN Yu1,2,Laurent SGALAT2   

  1. 1. School of Life Science, East China Normal University, Shanghai200062, China; 2. Center for Cell and Molecular Genetics, Université Claude Bernard Lyon 1, Lyon 69100, France
  • Received:2008-10-10 Revised:2008-11-27 Online:2009-11-25 Published:2009-11-25
  • Contact: QIAN Yu

Abstract: Protein kinase C regulates the progressive muscle degeneration in Caenorhabditis elegans. In order to investigate the function of PKC involved in muscle degeneration, this paper cloned a cDNA isoform of pkc-2 gene of C.elegans and constructed the recombinant plasmids pPD118.20-pKG63 containing the isoform. The new isoform was then further studied for gene expression pattern. Immunocytochemistry experiment showed that this cDNA isoform expressed in body-wall muscle cells and located near the dense body.

Key words: pkc-2 gene, clone, immunocytochemistry, Caenorhabditis elegans, pkc-2 gene, clone, immunocytochemistry