Journal of East China Normal University(Natural Sc ›› 2006, Vol. 2006 ›› Issue (2): 98-104,.

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Regulation and Tissue Specific Expression of VHA-c3 of Arabidopsis Thaliana(Chinese)

XU Ping, LI Xiao-fang, ZENG Wei-jun, WANG Shui-ping   

  1. School of Life Science, East China Normal University, Shanghai 200062, China
  • Received:2005-04-08 Revised:2005-07-07 Online:2006-03-25 Published:2006-03-25
  • Contact: WANG Shui-ping

Abstract:

Gene expression of VHA-c was detected in Arabidopsis silique, flower, leaf, bolt, root. However, the expression level of VHA-c3 was much higher in leaves than in the other tissues. Analysis of the putative promoter of VHA-c3 was carried out in tobacoo leave system. A series of the promoter mutants were constructed in which various extents of VHA-c3 promoter were placed upstream of the GUS gene in vector pC1305.1. The expression of GUS was analyzed by Agrobacterium-mediated transient expression system.It was found that a 2 812 bp promoter region has high gene expression capacity. And there is a high potential key cis-element for leaf-specific expression between 2812-2 234 bp region.

Key words: c subunit, VHA-c3, promoter, V-ATPase, c subunit, VHA-c3, promoter

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