Abstract: The spores of Cheilolejeunea ventricosa (Schiffn.) X.L.He were cultured on MS media (without acid and vitamin, pH=6.2, with 1% agar and 1% sucrose). The sporeling development and oil body ontogeny were observed. After a 55-day-long axenic culture, young plants were obtained successfully. Sodium hypochlorite solution was used to sterilize the capsules. Our results revealed that the sterilizing treatment with 0.1% Sodium hypochlorite solution for a period of 5 seconds was optimal. Oil bodies in primary protonemata are nearly homogeneous, but distinctly compound in the leaf cells of the primary leafy shoots. The spore germination and sporeling of Cheilolejeunea ventricosa belongs to Lejeunea type.

Key words: liverworts, oil body, spores, tissue culture, sporelin, Cheilolejeunea ventricosa, liverworts, oil body, spores, tissue culture, sporelin