Journal of East China Normal University(Natural Sc ›› 2007, Vol. 2007 ›› Issue (4): 112-118.

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High Throughput Screening Method of Identifying Antagonist for Glucagaon Receptor in Chinese Herbs(Chinese)

WAN Juan, XU Zhi-liang
   

  1. Key Laboratory of Brain Functional Genomics of Shanghai, School of Life Science, East China NormalUniversity, Shanghai 200062, China
  • Received:2006-03-01 Revised:2006-04-05 Online:2007-07-25 Published:2007-07-25
  • Contact: XU Zhi-liang

Abstract: This paper generated a cell-based functional assay for human glucagon receptor antagonist screening. The human glucagon receptor cDNA was cloned into a mammalian expression vector pCDNA3.1 (h Glucagon receptor /pCDNA3.1). This plasmid was co-transfected with a reporter gene construct (3×CRE/3×MRE/SRE-LUC) in HEK293 cells. Stable cell line expressing both human glucagon receptor and the reporter gene was selected for compounds screening. The assay condition was optimized. Our results demonstrated that this cell-based assay can be optimized for high throughput screening and applied to identify antagonist for glucagon receptor in Chinese Herbs.

Key words: reporter gene, antagonist, high throughput screening, glucagon receptor, reporter gene, antagonist, high throughput screening

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