Abstract: Studying the morphogenesis of Dictyostelium discoideum development, we found that there were distinct differences between the wild cells KAX-3 and the mutant cells AK127(LagC gene was knockout). The mutant cells AK127 was blocked at loose aggregation stage for lacking gp150 during developmental stages. However, the wild cells KAX-3 completed the whole developmental stages successfully. Three isoenzymes were obtained from developing cells of Dictyostelium discoideum which included a wild type cells(KAX-3) and a mutant type cells Compared these isoenzymes by gel electrophoresis, we found that two different esterases appreared in AK127 while only one in KAX-3. Furthermore, the content of malate dehydrogenase (MDH) is more in AK127 than in KAX-3 at different developing stage. At 14h during development, the content of glutamate dehydrogenase (GDH) in AK127 is higher than in KAX-3;but at 16h GDH in AK127 is lower than in KAX-3. From the results, we learned that the pattern of some isoenzymes were changed between wild cells and mutant cells during development stage, indicating some changes in the expression of some genes. So it seemed that gp150 was an important factor in development.

Key words: development, morphogenesis, SDS-PAGE, Glutamate dehydrogenase(GDH), Malate dehydrogenase(MDH), Estersase(Est), dictyostelium, development, morphogenesis, SDS-PAGE, Glutamate dehydrogenase(GDH), Malate dehydrogenase(MDH), Estersase(Est)