Abstract: This study aimed at analyzing transcription of RHD gene in erythroid cells and non-erythroid cells,and further comparing transcription of RHD gene in individuals with different expression levels of D phenotype.The technique of reverse transcriptase polymorphism chain reaction(RT-PCR) and cDNA sequencing was used to detect RhD mRNA of HL-60,K562,Jurkat,THP-1,HECF and white blood cells from ten unrelated individuals with different Rh phenotypes（2 CCDEe,2 CCDee,2 ccDee,2 CcDee,ccDEe and CcDEe）and reticulocyte from additional ten unrelated individuals with different Rh phenotypes(3 CcDEe,2 CCDEe,2 CCDee，2 CcDee and 1 CCDuee).Results showed except erythroid cells, RhD mRNA was absent in HL-60,Jurkat,THP-1,HECF and nuclear cells of peripheral blood.K562 had a normal RHD transcript.RhD cDNA of reticulocyte from different Rh phenotypes was complex and some absent exon 7, exon 9,exon 7～9,or exon 4～9,but all with normal RHD transcript.Conclusion Multiple RHD transcript is produced by alternative splicing.All these Rh messenger RNA isoforms existing in erythroid cells,such as reticulocyte and K562,absent in white blood cells.

Key words: mRNA, cDNA, RHD gene, mRNA, cDNA