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    J* E* C* N* U* N* S* 2006 Vol.2006
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    Studies in Contrast Spatial Structure Solutions for Singular Perturbation Problems(Chinese)
    NI Ming-kang;
    Journal of East China Normal University(Natural Sc    2006, 2006 (1): 1-12.  
    Abstract3266)            Save
    This paper surveys recent developments in studies of contrast spatial structure solutions of singularly perturbed problems, which have rise since the beginning of 1990's. It mainly introduces a series of work about step-type and spike-type solutions in ODE, including critical and uncritical cases. In the
    meanwhile, it introduces the latest research progress of contrast spatial structure of variation problems. The outline of contrast spatial structure solutions in PDE is also included.
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    (Chinese)
    Journal of East China Normal University(Natural Sc    2006, 2006 (1): 13-14,3.  
    Abstract2347)            Save
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    (Chinese)
    Journal of East China Normal University(Natural Sc    2006, 2006 (1): 15-16.  
    Abstract2542)            Save
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    (Chinese)
    Journal of East China Normal University(Natural Sc    2006, 2006 (1): 17-19.  
    Abstract2227)            Save
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    (Chinese)
    Journal of East China Normal University(Natural Sc    2006, 2006 (1): 20-22.  
    Abstract2388)            Save
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    The Periodic Solution for A Kind of Parabolic Perturbed Equations(Chinese)
    NI Ming-kang;GU Xi
    Journal of East China Normal University(Natural Sc    2006, 2006 (1): 23-30.  
    Abstract3347)            Save
    The periodic solution for a kind of parabolic perturbed equations including transposed item is discussed. The uniqueness of the solution is proved, and the asymptotic expansion of the solution and remainder estimation are also given.
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    On the Diophantine Equation x3+1=38y2(Chinese)
    DUAN Hui-ming
    Journal of East China Normal University(Natural Sc    2006, 2006 (1): 35-39,1.  
    Abstract2643)            Save

    This paper has proved that the Diophantine equation x3+1=38y2 has only integer solutions(x,y)=(-1, 0),(31,±28).

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    Research of Singular Perturbation Theory in East China Normal University(Chinese)
    华东师范大学 数学系;上海 000
    Journal of East China Normal University(Natural Sc    2006, 2006 (1): 35-34.  
    Abstract3261)            Save
    The growth and development of the research in the singular perturbation theory in the Department of Mathematics at East China Normal University is reviewed, uncovering historical facts in this research area in China. Some outstanding Chinese scientists, such as QIAN Wei-chang, GUO Yong-huai, QIAN Xue-sen and LIN Jia-qiao, have made pioneer contributions in this area.
    The Chinese Culture Revolution interrupted the research. In the late 1970s, after the end of the Culture Revolution, the section on research and teaching of differential equations in the department of mathematics of ECNU decided to make the theory and application of singular perturbation as a research direction. In the early 1990s the research is thriving under the efforts of the
    mathematicians in China. After then, however, was a recession period because of the lack of new researchers. But now, as a result of Chinese Government's continuously supporting of scientific researches, and outstanding mathematicians such as Ni Ming-kang have returned from abroad, the research of singular perturbation is becoming active again at our university and inChina.
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    Multi-periodic Solutions for A Class of Resonance Second Order Systems(Chinese)
    MENG Hai-xia;GUO Xiao-feng
    Journal of East China Normal University(Natural Sc    2006, 2006 (1): 40-44,1.  
    Abstract3066)            Save
    The existence and multiplicity of periodic solutions are obtained for a class of su-perquadratic resonance nonautonomous second order systems ü(t)+k2ω2u+▽F(t, u(t)) = 0, a.e.t ∈ [0,T] by Z2 version of the Mountain Pass Theorem on the condition that is unbounded.
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    A Characterization of the [[alpha]]-[[beta]] Generalized Inverse and Its Reverse Law(Chinese)
    DENG Bin;CHEN Guo-liang
    Journal of East China Normal University(Natural Sc    2006, 2006 (1): 45-51.  
    Abstract2820)            Save
    This paper deals with the case when the α-βgeneralized inverse A(-1)α-β is a linear transformation, in which case we established the characterization of the α-β generalized inverse, and we also give several sufficient conditions that the reverse law holds.
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    Stability of Single-Input Bilinear Sampled-data Systems with Quantization(Chinese)
    YU Hong-wang;WANG Zhi-ming
    Journal of East China Normal University(Natural Sc    2006, 2006 (1): 52-56.  
    Abstract3229)            Save
    The stability of a class of single-input bilinear sampled-data systems with quantization is investigated in this paper. The systems are uniformly globally exponentially stable by proposing a small feedback controller. Based on that,the effect of quantization on the bilinear sampled-data systems are discussed. And under the constrained quantization level, the same stability property of the systems is still concluded.
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    Flexibility of Embeddings of Wheel Graphs on the Torus(English)
    DENG Mo;REN Han
    Journal of East China Normal University(Natural Sc    2006, 2006 (1): 57-62,7.  
    Abstract2816)            Save
    In this paper, we investigate the flexibility of a wheel graph on S1 and determine the structures and the number of embeddings of wheel graphs on the torus. As their applications, the structures of embeddings of some generalized halin graphs (may be nonplanar) on the torus are also determined.
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    The Sequential Mesh Test for A Proportion(Chinese)
    PU Xiao-long;YAN Zhang-geng;MAO Shi-song;ZHANG Ying-shan;LI Yan
    Journal of East China Normal University(Natural Sc    2006, 2006 (1): 63-71.  
    Abstract3275)            Save
    This paper proposed a new sampling plan, the sequential mesh test, in order to overcome the disadvantages of the widely used Sequential Probability Ratio Test (SPRT). The main idea of the new plan is to divide a SPRT test problem into a series of subproblems by inserting a number of test points. The paper presented in detail how to realize the new plan, and showed that
    the new plan has a much better control of the sample number than SPRT does. Finally it showed that the new method is also more powerful than IEC 1123 the well-known international standard for sampling inspection.
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    A Finite Difference Method for Time-periodic Solutions of Nonlinear Parabolic Equations(English)
    WEI Wei;WANG Yuan-ming
    Journal of East China Normal University(Natural Sc    2006, 2006 (1): 72-79.  
    Abstract2806)            Save
    A finite difference method with accuracies of fourth order in space and second order in time is proposed for time-periodic solutions of a nonlinear parabolic boundary value problem. A monotone iterative scheme is developed for proving the existence and uniqueness of the periodic solutions of the nonlinear discrete scheme, and the computational algorithms for the periodic solutions are also provided. The convergence of the discrete scheme is discussed. The numerical results show the advantages of the method.
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    Application of Wu′s Method in Propositional Calculus(Chinese)
    LI Jing;YANG Zong-yuan
    Journal of East China Normal University(Natural Sc    2006, 2006 (1): 80-86.  
    Abstract3070)            Save

    A formal definition of proposition formulae was provided by context-free grammar,and the conversion from proposition formulae to polynomials was solved automatically. Using Wu′s Method, the paper presented another solution to the problem of theorem reasoning. In addition, a method for producing logical deduction was introduced.

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    Automated Geometric Theorem Search MethodBased on Numeric Computation(Chinese)
    XUE Li;HOU Xiao-rong
    Journal of East China Normal University(Natural Sc    2006, 2006 (1): 87-91.  
    Abstract2828)            Save

    A new method based on numeric computation for automated geometric theorem proving and discovering is shown. And a dynamic searching system of geometric theorem has been implemented,which can solve elementary geometric problems efficiently, including some non-constructive problems.

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    Research on A Content-based Secure Architecture Model(Chinese)
    XUE Mei;SUN Shu-feng;GU Jun-zhong
    Journal of East China Normal University(Natural Sc    2006, 2006 (1): 92-99.  
    Abstract2931)            Save

    Based on the smaller information granularity security, granule technology was presented in this paper, and the information content security architecture model was also given. The model-based prototype system exampled with protecting WORD documents was implemented.

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    LOM-Based Community P2P Learning Resource Sharing Model(Chinese)
    YANG Jing;GU Jun-zhong;XUE Mei
    Journal of East China Normal University(Natural Sc    2006, 2006 (1): 100-107.  
    Abstract3123)            Save

    This paper studies the resource sharing in Tele-learning systems.A community P2P learning resource sharing model is proposed and the resource sharing will be restricted in certain communities. The metadata of digital learning resources is stated by LOM standard. By means of distributed metadata management in structured pure P2P topology, the distributed storage and bandwidth can be taken full advantage. The constitution of community, the publication and retrieval of learning resources are specified in this paper as well as implement of the model.

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    Research on Dynamic Synchronization Technology of Webpage Database(Chinese)
    XU Wen-tao
    Journal of East China Normal University(Natural Sc    2006, 2006 (1): 108-115.  
    Abstract3416)            Save

    There are plenty of local copies of pages of remote web sites on local databases on most of web search engine sites. It is necessary to pull remote web pages periodically to refresh local copies of these pages on database in order to keep copies and source pages consistent,and which takes plenty of time and resources.The article proposes serveral policies to synchronize copy and source pages , proposes a Poission model of source page change, define freshness and fresh time, studies on these policies and compares their effectiveness.It is shown that the proposed policies improve the freshness of web pages significantly.

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    Silver Nano-particles Synthesized on Porous Silicon by Thermal Decomposition(Chinese)
    FENG Xiao;XU Shao-hui;ZHU Zi-qiang
    Journal of East China Normal University(Natural Sc    2006, 2006 (1): 116-119.  
    Abstract2827)            Save

    Uniform Ag nano-particles was synthesized within nanoscale pores of oxidized porous silicon (OPS) by a simple solution immersion-thermal decomposition approach,forming the compound structure of Ag/OPS/silicon. Ag films deposited on OPS were characterized by X-ray diffraction, and SEM showed the existence of silver nano-particles.

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    Analyzing of Quantum Information Experiment in Operator(Chinese)
    ZHANG Zhi-jun;LI Jin-fang;MA Lei
    Journal of East China Normal University(Natural Sc    2006, 2006 (1): 120-125.  
    Abstract2775)            Save

    In this paper, we defined lowering and raising operator of single photon's two polarization states, and used the theory of linear transformation of operator in Fock space to study BS, PBS, wave plate and H transformation. We obtained their representation of operator .On the base of this; we studied some quantum information experiment, e.g. Teleportation, from a new aspect. Using the new representation, we can analyze the probability of photons output states .To do this, we try to find a new method to devise experiments much efficiently.

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    Differential Algorithm in Wave-height Measurement(Chinese)
    LI Yi-xiang;ZHENG Zheng-qi;JIANG Song-ying;KANG Wei
    Journal of East China Normal University(Natural Sc    2006, 2006 (1): 126-131.  
    Abstract3001)            Save

    This paper advances a novel measurement of wave-height — differential algorithm.Compared with electric level method, the differential algorithm has its advantages. This article details its principle,algorithm and demonstrates it with a living example.

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    (Chinese)
    Journal of East China Normal University(Natural Sc    2006, 2006 (1): 132-135.  
    Abstract2280)            Save
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    (Chinese)
    Journal of East China Normal University(Natural Sc    2006, 2006 (1): 136-139.  
    Abstract2379)            Save
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    Syntheses and Biological Activity of Novel Heteroretinoids(Chinese)
    CHEN Zhen;WANG Jing;XU Zi-jin;ZOU Xin-zhuo
    Journal of East China Normal University(Natural Sc    2006, 2006 (2): 1-6.  
    Abstract2896)            Save

    Syntheses of three novel heteroretinoids were reported. Novel heteroretinoids were 5-(5,6,7,8-Tetrahydro-5,5,8,8-tetramethyl-2-naphthalenyl)-3-pyrazole carboxylic acid, 2-Methyl -5-(5,5,8,8-tetramethyl-5,6,7,8-tetrahydro-naphthalen-2-yl)-furan-3-carboxylic acid, 1-Butyl -2-methyl-5-(5,5,8,8-tetramethyl-5,6,7,8-tetrahydro-naphthalen-2-yl)-1H-pyrrole-3-carboxylic acid.The results of biological test indicated that these compounds possessed some growth inhibition effects in leukemia cells.

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    Determination of Content of RNA in Yeast by Capillary Zone Electrophoresis with Amperometric Detection(Chinese)
    CHEN Xiang;WANG Qing-jiang;DING Fei;LU Jin;HE Pin-gang;FANG Yu-zhi
    Journal of East China Normal University(Natural Sc    2006, 2006 (2): 7-12.  
    Abstract3307)            Save
    The indirect determination of the content of RNA in yeast by analyzing adenine (A) and guanine (G) with capillary electrophoresis with amperometric detection (CE-AD) was reported. The effects of potential of working electrode, running buffer, separation voltage and injection time on the CE-AD determination were investigated. Adenine and guanine could be well separated in a 30 mmol/L borate buffer (pH 9. 0) within 18 min under the optimum conditions. Their detection limits of adenine (S/ N = 3) of 2.0 ×10-7 mol/ L and 1.7 ×10 -7mol/ L, respectively. The experimental results showed that this method is of high sensitivity and high repeatability, and potential to be used in the quick analysis of yeast in foods.
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    Determination of Sinapic Acid,Quercetin and Protocatechuic Acid in Allium cepa Linn by Capillary Zone Electrophoresis with Electrochemical Detection(Chinese)
    GENG Cheng-huai;CHU Qing-cui;YE Jian-nong
    Journal of East China Normal University(Natural Sc    2006, 2006 (2): 13-17.  
    Abstract3365)            Save
    A simultaneous determination of sinapic acid, quercetin and protocatechuic acid in Allium cepa Linn by capillary aone electrophoresis with electrochemical detection was reported. The parameters affecting the separation and detection such as detection potential, separation voltage and concentration of running buffer were evaluated. Under the optimum conditions, the analytes were baseline separated within 25 min in a borax buffer (PH=9.0). Good linear relationship was established between peak current and concentration of analytes in the range of 2.0×10-7-1.0×10-4g/mL for sinapic acid, 2.0×10-7-5.0×10-5g/mL for quercetin, and 5.0×10-7-5.0×10 -5g/mL for protocatechuic acid. The detection limit (S/N=3) is 1.5×10-7g/mL,1.6×10-7g/mL and 3.6×10-7g/mL respectively for these three analytes. This method has been used for the determination of active ingredients in allium cepa linn with satisfactory results.
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    Analysis of Remote Sensing Image Format —— GeoTIFF(Chinese)
    CHEN Duan-wei;SHU Jiong;WANG Qiang;DUAN Yu-sen
    Journal of East China Normal University(Natural Sc    2006, 2006 (2): 18-26.  
    Abstract7281)            Save
    GeoTIFF, a geographically extended image format of TIFF, was studied in this paper. Description and analysis were given to TIFF format, storing methods of GeoKeys, three kinds of coordinate systems supported by GeoTIFF, six GeoTags and their relationships, as well as coordinate transformation and geocoding. Advantages of GeoTIFF were explained, and its application prospect is anticipated.
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    Component GIS for Census in Pudong New Area(Chinese)
    WANG Yuan-fei;ZHOU Feng;LIU Zhi-qiang;MI Wei-jie;LU Tao;DING Jing-hong
    Journal of East China Normal University(Natural Sc    2006, 2006 (2): 27-32.  
    Abstract3625)            Save
    A census GIS, which named as Pudong New Area Census GIS (PucGIS), was developed using techniques of component GIS, data ware house, demographic and population modelling. The detailed demographic index was designed in the system for exploring more information from census database. The technology of data ware house was used in census database designing, Pudong New Area census data was partitioning in spatially by the census block, which coincide with the political boundary of Jiedao or Zheng. This two level database improving the execution efficiency of PucGIS greatly.
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    Analysis on the Relationship between Heavy Rainfall and Features of Environmental Fields(Chinese)
    DAI Xiao-yan;GUO Zhong-yang;SU Jun-yi;GU Xin-ye
    Journal of East China Normal University(Natural Sc    2006, 2006 (2): 33-40.  
    Abstract3049)            Save
    Hong Kong Observation Operational Regional Spectral Model (ORSM) forecasting physical values in June and July 2002 were used to explore the large-scale atmosphere circulation situations and physical conditions causing continuously severe rainfall in the south of China by analyzing the features of average geopotential height field, temperature field, humidity field, meridional and zonal wind velocity field when heavy rainfall took place. The study of the thermodynamic and dynamical field near the heavy rainfall belt indicates that in temperature field, at the low and middle level of atmosphere, the heavy rainfall belt lies in the comparatively colder zone south of the high temperature zone with lower gradient, while the gradient is higher in the north of the heavy rainfall belt. On the other hand, in humidity field, the heavy rainfall belt is located in the high humidity zone, while at the low level of atmosphere, it is in the south of relative humidity gradient zone. Furthermore, in wind velocity field, at the low level of atmosphere, the heavy rainfall belt existed in the convergence zone of the east and west winds, south and north winds.
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    Characteristics of Hydrology and Sediment Transportation and Influence by Reservoir Project in the Meimaosha of Yangtze Estuary(Chinese)
    ZUO Shu-hua;LI Jiu-fa;YING Ming;SHI Lian-qiang;XU Hai-gen
    Journal of East China Normal University(Natural Sc    2006, 2006 (2): 41-48.  
    Abstract3723)            Save
    Using the data of years, including the latest in situ data observed in Meimaosha and adjacent water areas in the southern passage of the Yangtze Estuary in Sep., Dec. 2003 and Jan. 2004, characteristics of hydrology and sediment were studied by the multi-methods of hydrology, sediment transportation and statistics. The results show that tidal currents show a strong ebb dominance as velocity of the ebb current is larger than the flood current, time of the ebb current longer than the flood current. In general, the average suspended sediment concentration is 1.40 kg/m3 during the period of the spring tide and 0.60 kg/m3 during the period of the neap tide respectively. The observed data also show that suspended sediment concentration during the flood season is higher than that of during the dry season. Suspended sediment concentration of the flood tide is higher than the ebb tide in the spring tide during the flood season, which is contrary in the neap tide. During the dry season either in the spring tide or in the neap tide, suspended sediment concentration of the ebb tide is higher than the flood tide. The change of the suspended sediment concentration gradient is evident. The variety of the current velocity and suspended sediment concentration after the ecotypic reservoir project have been constructed with the current continuity equation and a linear regression formula of sediment carrying capacity. The result show that it will affect the Nanhui tidal flat in a certain extent.
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    Differentiation of the Ciliature and the Base-Associated Fibers of Onychodromus quadricornutus during Asexual Divisions(Chinese)
    Zhu Hui;Zou Shi-fa;Ni Bing;Gu Fu-kang
    Journal of East China Normal University(Natural Sc    2006, 2006 (2): 49-57.  
    Abstract3136)            Save
    The differentiation of the ciliature and the base-associated fibers during asexual division of the fresh water ciliate, Onychodromus quadricornutus (Foissner et al. 1987) were observed by using protargol staining technique. During asexual division of O. quadriconutus, AZM and PM of the proster is formed at the site where the old oral structures is located and there is no detectable close relations between the new and old structures during the morphogenesis of left and right marginal cirri, the phenomena different from what found in some known oxytrichids. The cirri base-associated fibers of O. quadriconutus include anterior and posterior longitudinal fibers, transverse fibers and radiating fibers. The appearance of these fibers are similar to that observed in some known ciliates, except for that of the transverse fibers of left marginal cirri which differs from the known ciliates in location. During morphogenesis, the old ciliatures together with their base-associated fibers begin to degenerate and disappear in the primordial zone of new anterior and posterior FTV cirri. The base-associated transverse fibers of the old left and right marginal cirri are observed to be the first to disintegrate and degenerate. The disintegration of the base-associated transverse fibers in these parts of old structures shows a close relation with the differentiation of the primordial zone, suggesting an important role of this disintegration in supplying differentiation and development of the primordium with some substances. Other old ciliatures and the base-associated fibers can remain for a relative longer time in the cortex and finally begin to degenerate and disintegrate when their function declines gradually.
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    Effects of Symbiont on Saline Tolerance and Isozymes of Host in Paramecium bursaria-Chlorella System(Chinese)
    SUN Jun;ZHANG Ying;GU Fu-kang
    Journal of East China Normal University(Natural Sc    2006, 2006 (2): 58-62.  
    Abstract2982)            Save
    To further investigate the effect of symbiotic Chlorella on physiological process and metabolism of the host P. bursaria, NaCl or Pb(NO3)2 tolerance and 12 types of isozyme of Chlorella-containing P. bursaria(CCP) and recovered Chlorella-containing P. bursaria(RCCP) and Chlorella-free P. bursaria(CFP) were analyzed. The results showed that NaCl tolerance of CCP was similar to that of RCCP, different from that of CFP, and that Pb(NO3)2 tolerance and activity of isozymes of CFP were similar to those of RCCP. RCCP showed the transitional characteristics between CCP and CFP. Diverse composition and activity of isozymes of among the three samples indicated existence or symbiotic time of Chlorella could change the isozyme system of the host. Fewer composition and lower activity of isozyme in CCP probably implied that due to the symbiosis of Chlorella, isozyme system of the host degenerated in “P. bursaria - Chlorella” system.
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    Function of gp150 in the Differentiation of Stalk Cell during〖WTHX〗 Dityosteliun Discoideum Development(Chinese)
    HOU Lian-sheng
    Journal of East China Normal University(Natural Sc    2006, 2006 (2): 63-67.  
    Abstract3477)            Save
    Cells of Dictyostelium discoideum are trigged to undergo development when they are depleted their food source. Cell adhesion molecules play an important role in maintaining the integrity of cell aggregation and influencing subsequent cell-type differentiation and cell sorting. At the loose aggregation stage , gp150 was localized on the peripheral area of multicellular aggregation.At the slug stage, the prespore cell domain and prestalk cell domain were separated by the gp150 molecule, quantity of the gp150 molecule become to increase in the pretalk cell domain following Dictyostelium discoideum development. Thus the temporal of gp150 molecule expression and the special localization of gp150 molecule are related with the differentiation of stalk cells Dictyostelium discoideum development.
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    Optimization of PCR Conditions for Macrobrachium nipponensis Gene Fragments(Chinese)
    ZHAO Xiao-qin;NI Juan;CHEN Li-qiao;GU Zhi-min;ZHOU Zhi-ming
    Journal of East China Normal University(Natural Sc    2006, 2006 (2): 68-74,8.  
    Abstract3285)            Save

    Genomic DNA was isolated from the musculature of Macrobrachium nipponensis to establish a reproducible and stable PCR technical system. The ingredients and programs of amplified reactions were optimized respectively. The results showed that the profiles of PCR products could be improved obviously by using the 25 μL solution of 10×Buffer 2.5 μL, Mg+2 1.5 mmol/L, 4×dNTP 0.2 mmol/L, 1 U Taq DNA polymerase, 0.4 μmol/L of each primer and 200~300 ng of DNA template as well as the programs of 5 min at 95 ℃, 35 cycles of 1 min at 95 ℃, 50 s at 56 ℃, 1 min at 72 ℃ and finally, 10 min at 72 ℃. The optimized conditions could result in satisfactory specificity and reproducibility in amplifying 18S rRNA,COI,ND5 gene fragments, and consequently provide a useful means for the research of genetic diversity, molecular marker and germplasm identification in Macrobrachium nipponensis.

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    Digestive Enzyme Activities and the Contents of the Amino Acid during the Embryonic Development of Macrobrachium nipponense(Chinese)
    ZHAO Yan-min;ZHAO Yun-long;Wang Qun;YAO Jun-jie;AN Chuan-guang;DUAN Xiao-wei
    Journal of East China Normal University(Natural Sc    2006, 2006 (2): 75-81.  
    Abstract3288)            Save

    Amino acids composition and activities of four digestive enzymes including pepsin, tryptase, amylase, cellulase were determined in the eggs of freshwater prawn, Macrobrachium nipponense. These eggs were sampled from different embryonic development stages. The results indicated that digestive enzymes activities and amino acids correlated closely with the stages of embryonic development. Activities of pepsin, tryptase and amylase were relatively high during the whole embryonic development stages. Activities of pepsin, tryptase and amylase were high in early and late developmental stages, but relatively low during middle stages. Cellulose activity was low and showed no obvious change during different embryonic development stages. During protozoea stage, activities of pepsin, tryptase and amylase increased obviously. The content of dissolvable protein and amino acid had a decreased trend. It had relevance to the activities of related enzymes. However, content of the dissolvable protein and amino acid during gastrula stage were higher than that of during blastula stage. Among all the amino acids determined, content of Glu was the highest, the highest essential amino acid (EAA) content was Leu. There was little difference between the ratios of content of single EAA to total EAA during different stages of embryonic development of M. nipponense.

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    Comparative Study of Trypsinase Digestion and Homogenization in Acquiring Free Sperms from Eriocheir Sinensis(Chinese)
    MA Qiang;WANG Qun;LI Kai;DING Ying-di
    Journal of East China Normal University(Natural Sc    2006, 2006 (2): 82-87.  
    Abstract2976)            Save

    The purpose of this paper was to compare two methods of acquiring free sperms from spermatophores of Chinese mitten-handed crab (Eriocheir sinensis). Under the condition of pH 7.4 and different reaction temperatures (27 ℃,32 ℃,37 ℃,42 ℃and 47 ℃, respectively), trypsinase concentrations (0.05 %, 0.10 %, 0.15 %, 0.20 % and 0.25 %, respectively) and reaction times(5 min,10 min,15 min,20 min and 25 min, respectively), the best reaction condition of trypsinase digestion method (TDM) was studied by orthogonal tests and the sperm amounts which got from each gram of spermatophores was used as a criterion. According to the results of orthogonal tests, we found that enzymatic reaction time was the primary factor, and the reaction temperature and the trypsinase concentration also functioned. The maximum effect of trypsinase digesting works at temperature 37 ℃, trypsinase concentration 0.20 % and time 5 min. By using TDM the amounts of free sperms acquired from each gram of spermatophore were 7.41×108 which was significantly higher than that using HM which was only 3.33×108 and the sperm mortality rate was 4.75 % by using TDM which was significantly lower than that using HM which was 27.00 %. Furthermore, the acrosome reaction ability was detected by artificially-induced methodology and the reaction rates were 62.3 % (TDM) and 64.5 % (HM), and there was no significant difference between them. These results suggest that TDM is more effective than HM in acquiring free sperms.

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    15α-Hydroxylation of 13-ethy1-estr-4-ene-3,17-dione Using Immobilized Penicillium raistrickii by Calcium Alginate gel Embedding Methd(Chinese)
    LI Jun;GENG Xu;ZHANG Pei-jun;WU Zi-rong
    Journal of East China Normal University(Natural Sc    2006, 2006 (2): 88-92.  
    Abstract2643)            Save

    15α-hydroxylation of 13-ethyl-estr-4-ene-3,17-dione using immobilized Penicillium raistrickii entrapped in calcium alginate gel was studied. The conversion conditions, which affected hydroxylation such as substrate concentration, the time to add substrate, conversion duration, were investigated. The results show that, the immobilized Penicillium raistrickii can be utilized repeatedly five times with no obvious decline of hydroxylation ability.

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    Effect of Puararia Flavonoids on AGEs Induced Bovine Vascular Endothelial Cells Injury in Vitro(Chinese)
    XU Min-hua;YE Xi-yun;ZHANG Long;WANG Yao-fa
    Journal of East China Normal University(Natural Sc    2006, 2006 (2): 93-97.  
    Abstract2783)            Save

    The effects of Puararia flavonoids(PF) on bovine vascular endothelial cells(VEC) in vitro injured by advanced glycation end products(AGEs) were investigated. When VEC was incubated with AGEs(4 g/L) for 48 hours, the growth of VEC was enhanced, the contents of lactate dehydrogenase(LDH) and malondialdehyde(MDA) and lipofuscin(LPF) were markedly increased(P<0.01), the contents of nitric oxide(NO-2/ NO-3) and glutatuhione(GSH) were reduced. Treatment with PF caused a reduction in LDH and MDA and LPF, the contents of GSH and NO-2/ NO-3 were increased. The results suggest AGEs has direct effects on VEC, perhaps oxidative stress participates in this process. PF can protect cultured VEC from being injured by AGEs, thereby exert favorable effect against the occurrence and development of chronic vascular complications in diabetic mellitus.

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    Regulation and Tissue Specific Expression of VHA-c3 of Arabidopsis Thaliana(Chinese)
    XU Ping;LI Xiao-fang;ZENG Wei-jun;WANG Shui-ping
    Journal of East China Normal University(Natural Sc    2006, 2006 (2): 98-104,.  
    Abstract3039)            Save

    Gene expression of VHA-c was detected in Arabidopsis silique, flower, leaf, bolt, root. However, the expression level of VHA-c3 was much higher in leaves than in the other tissues. Analysis of the putative promoter of VHA-c3 was carried out in tobacoo leave system. A series of the promoter mutants were constructed in which various extents of VHA-c3 promoter were placed upstream of the GUS gene in vector pC1305.1. The expression of GUS was analyzed by Agrobacterium-mediated transient expression system.It was found that a 2 812 bp promoter region has high gene expression capacity. And there is a high potential key cis-element for leaf-specific expression between 2812-2 234 bp region.

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