Expression analysis of Brassica napus L. droughtrelated genes under drought stress
2016, 2016 (1):
The high droughttolerant variety Holiday was treated as the experimental material under drought stress in the early flower stage. The expression level of 6 droughtrelated genes (ABA2、BnSOS2、BnCS、 CAM、CBF4、PIP1) which were selected from different organs (root, stem, leaf, flower and green pod) at different stress times (1,3,5,7 days) were analyzed by qRT PCR technology. The result showed that the expression of six droughtresistant genes in different organs all increased under drought stress, and they presented different trends respectively under different drought stress. In the same organs, the expression of the six droughtresistant genes had obvious different. In root, the maximum cumulative expression value gene was ABA2, and the minimum cumulative expression value gene was CBF4; in stem, the maximum was CAM, the minimum was CBF4; in leaf, the maximum was PIP1, the minimum was ABA2; in flower, the maximum was CBF4, the minimum was BnSOS2; in green pod, the maximum was BnCS, the minimum was CBF4. It suggested that different drought resistance pathway played〖JP2〗 different role under drought stress. The correlation analysis between expression values of droughtrelated genes and stress time showed that the expression quantity of CAM gene in the stem volume, CBF4 gene in the flowers was significantly positively related to the stress time.
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