Expression analysis of Brassica napus L. droughtrelated genes under drought stress

doi:10.3969/j.issn.10005641.2016.01.015

Abstract

Abstract: The high droughttolerant variety Holiday was treated as the experimental material under drought stress in the early flower stage. The expression level of 6 droughtrelated genes (ABA2、BnSOS2、BnCS、 CAM、CBF4、PIP1) which were selected from different organs (root, stem, leaf, flower and green pod) at different stress times (1,3,5,7 days) were analyzed by qRT PCR technology. The result showed that the expression of six droughtresistant genes in different organs all increased under drought stress, and they presented different trends respectively under different drought stress. In the same organs, the expression of the six droughtresistant genes had obvious different. In root, the maximum cumulative expression value gene was ABA2, and the minimum cumulative expression value gene was CBF4; in stem, the maximum was CAM, the minimum was CBF4; in leaf, the maximum was PIP1, the minimum was ABA2; in flower, the maximum was CBF4, the minimum was BnSOS2; in green pod, the maximum was BnCS, the minimum was CBF4. It suggested that different drought resistance pathway played〖JP2〗 different role under drought stress. The correlation analysis between expression values of droughtrelated genes and stress time showed that the expression quantity of CAM gene in the stem volume, CBF4 gene in the flowers was significantly positively related to the stress time.

Key words: Brassica napus L. (rapeseed), drought stress, drought related genes, gene expression, quantitative realtime PCR

CLC Number:

S565.4

HE Qiao-Li, ZHANG Zhe, RAN Chun-Yan, XIE Xiao-Yu. Expression analysis of Brassica napus L. droughtrelated genes under drought stress[J]. Journal of East China Normal University(Natural Sc, 2016, 2016(1): 113-122.

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