Journal of East China Normal University(Natural Sc ›› 2019, Vol. 2019 ›› Issue (3): 164-173.doi: 10.3969/j.issn.1000-5641.2019.03.018

• Ecological and Environmental Science • Previous Articles     Next Articles

Community structure and gene expression analysis for nitrifier enrichment cultures

YU Chen-di1, HOU Li-jun1, ZHENG Yan-ling2,3, LIU Min2,3, YIN Guo-yu2,3, GAO Juan1, LIU Cheng1, CHANG Yong-kai1   

  1. 1. State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai 200241, China;
    2. Key Laboratory of Geographic Information Science(Ministry of Education), East China Normal University, Shanghai 200241, China;
    3. School of Geographic Sciences, East China Normal University, Shanghai 200241, China
  • Received:2018-03-02 Online:2019-05-25 Published:2019-05-30

Abstract: Nitrifiers were enriched from the tidal sediments of the Yangtze Estuary. Four kinds of nitrifying groups (accounting for 34.7% of the total reads) were detected based on metagenomics, including the recently discovered complete ammonia oxidizers (comammox), strictly nitrite-oxidizing Nitrospira, β-proteobacterial ammonia-oxidizing bacteria, and ammonia-oxidizing archaea. Comammox accounted for 48% of the detected nitrifiers. In addition, gene expression patterns of the nitrifier group (according to the classification in Evolutionary genealogy of genes:Non-supervised Orthologous Groupsdatabase) were analyzed based on metatranscriptomics. The present study showed the community structure and gene expression characteristics of nitrifiers enriched from the tidal sediments, especially the newly discovered comammox. Results enriched our knowledge about the nitrifying communities of estuarine environment, which have great significance to the further molecular ecology research of the nitrification processes.

Key words: nitrifier, enrichment culture, tidal sediment, community structure, gene expression

CLC Number: