Journal of East China Normal University(Natural Science) ›› 2023, Vol. 2023 ›› Issue (3): 118-131.doi: 10.3969/j.issn.1000-5641.2023.03.012

• Estuary and Coastal Research • Previous Articles    

Metabolic potential and environmental adaptation mechanisms of Nitrospira in tidal flat wetlands

Guohao CHEN, Tieqiang MAO, Hongpo DONG*(), Yafei OU, Jiawei ZHANG   

  1. State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai 200241, China
  • Received:2021-07-26 Accepted:2021-11-25 Online:2023-05-25 Published:2023-05-25
  • Contact: Hongpo DONG


To understand the metabolic potential and environmental adaptation mechanisms of Nitrospira in tidal flat wetlands, 14 high-quality Nitrospira genomes were constructed from five tidal flat wetlands along the coast of China using metagenomic binning and assembly methods. Phylogenetic analysis showed that among these genomes, three belonged to Comammox (complete ammonia oxidizer), nine belonged to lineage II and IV of Nitrospira, and two belonged to lineage III, which has yet to be discovered; taken together, this data suggests that abundant and diverse Nitrospira are present in China’s tidal flat wetlands. Metabolic reconstruction revealed that these Comammox and Nitrospira contained cyanase, urease, and other enzymes involved in the degradation of nitrile compounds and amide compounds; hence, they may utilize the organic nitrogen as energy by coupling with ammonia-oxidizing microorganisms. In addition, Nitrospira possessed flexible strategies to resist environmental stresses such as viral attack and osmotic changes. These results provide insights on the diversity, ecological function, and environmental adaptation mechanisms of Nitrospira from tidal wetlands.

Key words: Nitrospira, phylogeny, metabolic potential, environmental adaptation, tidal flat wetland

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