Abstract: A novel chemically modified ultramicroelectrode os described for the detection of cytochrome Cin this paper. Firstly, ethidene diamine(ED) was eletropolymerized on the surface of carbon fiber ultramicroelectrode(UME),and then gold colloid nano particles was deposited on the surface(Au/ED/UME). At last, we dipped the Au/ED/UME into the potassium iodide solution and then the iodide ion was absorbed onto the surface of Au/ED/UME. The electrochemical behavior of the prepared modified I^-/Au/ED/UME was studied. The chemically modified UME, which was used as working electrode, has good electrocatal reduction of cytochrome c with a detection limit of 3.2×10^-7mol/L and a linear concentration range of 6.5×10^-7~1.2×10^-5mol/L(vs.SCE). We think it has great potential for application for in vivo studying of cytochrome C.

Key words: Carbon fiber modified ultramicroelectrode, cytochrome C., Gold colloid nano particles, Carbon fiber modified ultramicroelectrode, cytochrome C.