Abstract:

In order to study the relationship between vegetative cell and resting cyst, RAPD method was selected. The result revealed that: the polymorphic fragments amplified with primers S176、S267、S217、S259 and S319 share the same fragments, but the other primers such as S283、S180、S61、S64、S17、S55、S38、S30、S23 and S28 have deferent amplied fragments. There are 84 fragments were amplified by 15 random primers. In these fragments, both vegetative cell and resting cyst mtDNA have 28 similar fragments. The similarity values is 66.67%. It is implied that though there is similarity between mtDNA of vegetative cell and resting cyst, some changes may occurred in resting cyst mtDNA, these changes may be related to the function of resting cyst mitochondrion.

Key words: RAPD, cyst, Pseudourostyla cristata, mtDNA, RAPD, cyst, Pseudourostyla cristata