Journal of East China Normal University(Natural Sc ›› 2003, Vol. 2003 ›› Issue (3): 61-68.

• Article • Previous Articles     Next Articles

Study on Size Variation of rDNA Internal Transcribed Spacer 1 in Eriocheir japonica sinensis (Decapoda,Grapsidae)

TANG B0-ping123;ZHOU Kai-ya2; SONG Da-xiang2; CHEN Li-qiao1   

  1. 1 College of Life Science, East China Normal University, Shanghai 200062. China 2 College of Life Science, Nanjing Normal University, Nanjing 210097. China 3 Department of Biology, Yancheng Teachers College,Yancheng 224002 China
  • Received:2002-09-16 Revised:2002-12-08 Online:2003-09-10 Published:2003-09-10

Abstract: The intra-individual size variation of rDNA ITS1 (first internal transcribed spacer of rDNA) was detected in some invertebrates, such as Schistoaoma japonicum, Paragonimus westermani, Simulium. In our experiment of investigating the primary structure of ITS1 of Eriocheir japonica. sinensis the intra-individual size polymorphism was detemined at first, but further study showed that the polymorphism was an artifact,which was due to PCR primers mismatching.This conclusion was finally demonstrated by verifying conditions of PCR and reamplifying the ITS1 domain using redesigning primer that was corresponding to 5.8S rDNA sequences of E. japonica. sinensis that obtained in this study. The nucleotide sequence of the ITS1 of E.japonica sinensis has been firstly determined in this species.

Key words: ITS1, size variation, decapoda, Eriocheir japonica sinensis, ITS1, size variation, decapoda