Journal of East China Normal University(Natural Sc ›› 2008, Vol. 2008 ›› Issue (2): 53-58,9.

• Article • Previous Articles     Next Articles

Differential expression analysis of the wild cells KAx-3 and the mutant cells AK127 in Dictyostelium discoideum(Chinese)

WANG Yi-zheng,ZHANG Min,HOU Lian-sheng   

  1. School of Life Science, East China Normal University, Shanghai200062, China
  • Received:2006-10-11 Revised:2006-11-15 Online:2008-03-25 Published:2008-03-25
  • Contact: HOU Lian-sheng

Abstract: To study the influence of gp150 protein on the gene expression during Dictyostelium discoideum development, differential display was used to analyze the differences of gene expression between the wild cells KAx-3 and the mutant cells AK127 (absence of gp150) at 14 h of their development. The results indicated that gene expressions are obviously different between the two type cells. The 275 bp fragments which did not express in the mutant cells showed partial sequence similar to the gene encoding histidine kinase, STATc protein and homeobox protein. The data suggest that mutant cells cannot complete development because gp150 is responsible for the mediation of the gene expression of some regulatory factor

Key words: gp150 protein, mRNA differential display, Dictyostelium discoideum, gp150 protein, mRNA differential display

CLC Number: