Research on separation method of highly polar compound DMC and impurities in it by HILIC

Abstract

Abstract: HILIC method was established to separate and analyze highly polar compound methacryloyloxyethyl trimethyl ammonium chloride (DMC) and its impurities. Using two different chromatographic columns：HP-Cyano column and X-Bridge HILIC column，highly polar compound DMC and its impurities were all well separated by gradient elution using acetonitrile and water as mobile phase. With the two different chromatographic columns, four impurities in DMC were separated, such as dimethylaminoethyl methacrylate (DM),methacrylic acid (MAA), choline chloride (CC) and Hydroquinone monomethyl ether (MQ). This method has higher practical value, which can be used effectively in separated highly polar compounds.

Key words: hydrophilic interaction liquid chromatography (HILIC), methacryloyloxyethyl trimethyl ammonium chloride (DMC), highly polar compounds, separation

CLC Number:

O657.7+2

HAN Bing-bing, WANG Cui-cui, LI Xin-jun, ZOU Xin-zhuo. Research on separation method of highly polar compound DMC and impurities in it by HILIC[J]. Journal of East China Normal University(Natural Sc, 2011, 2011(6): 132-138.

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