Journal of East China Normal University(Natural Sc ›› 2013, Vol. 2013 ›› Issue (5): 53-60.

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Microtubular organelles in Euplotes aediculatus (Ciliophora: Hypotrichida) revealed by fluorescent labeling

LIN Qin, FAN Xin-peng, SUN Lei, ZHOU Su-juan, GU Fu-kang   

  1. School of Life Sciences, East China Normal University, Shanghai 200062, China
  • Received:2012-05-01 Revised:2012-08-01 Online:2013-09-25 Published:2013-09-30

Abstract: The cortical ciliature microtubules, base-associated microtubules, the dorsal cortical microtubule structure, as well as the morphogenesis of the cortical ciliature microtubules in Euplotes aediculatus were analyzed with FLUTAX (fluorescence taxoid) and anti-α-tubulin antibody immuno-fluorescence labeling. The results indicate that the dorsal cortical network which is called silver-line system morphologically associates with microtubular cytoskeleton. Moreover, differing from other Euplotes, The morphogenesis mode of the adoral ciliature microtubules and dorsal cortex ciliature microtubules in the proter of E. aediculatus represent a distinct morphogenesis mode which might be a referable basis for species differentiation. Additionally, anti-α-tubulin antibody immuno-fluorescence labeling shows dorsal cortex network and the early frontal-ventral-transverse cirri primordia more clearly comparing with fluorescently labeling by FLUTAX. This might suggest the tubulin element is different between cell dorsal cortex surface network and other microtubular structures, and the tubulin element may change gradually as the assembling and maturing of cortical ciliature microtubule in sequential morphogenesis stages.

Key words: ciliate, Euplotes aediculatus, fluorescent labeling, cortical ciliature microtubule, dorsal cortex surface network

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