Journal of East China Normal University(Natural Sc ›› 2019, Vol. 2019 ›› Issue (2): 156-163.doi: 10.3969/j.issn.1000-5641.2019.02.017

• Chemistry and Chemical Engineering • Previous Articles     Next Articles

Determination of sulfonamides by ultra-high performance liquid chromatography with microextraction

LI Yi, ZHANG Yan, ZHANG Ya-ting, HE Pin-gang, WANG Qing-jiang   

  1. School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China
  • Received:2017-12-04 Online:2019-03-25 Published:2019-03-27

Abstract: In this study, the development of an analytical method for the determination of chicken meat based on three-phase hollow fiber membrane microextraction ultra-high performance liquid chromatography (HF-LPME-UHPLC) was explored. A three-phase hollow fiber membrane microextraction system was developed to optimize the extraction conditions of HF-LPME:the extractant was n-octanol, the concentration of NaOH in the receiving phase was 200 mmol/L, the concentration of hydrochloric acid in the sample solution was 1.0 mmol/L, the concentration of Na2SO4 was 375 g/L, the extraction temperature was 30°, the stirring rate was 300 r/min, and the extraction time was 4 hours. Chicken meat samples were extracted and analyzed by UHPLC. Chromatographic analysis showed that the six sulfonamides reached baseline separation within six minutes. The linearity of the six sulfonamides in the mass range was good with a correlation coefficient of R2 > 0.998 9, the enrichment factors ranged from 188 to 950 times, and the detection limit was 0.03~0.1 ng/mL (S/N=3). The recoveries of sulfonamides flagged in chicken were 85.2%~95.6% with relative standard deviations of 3.13%~4.33% (n=5) at a concentration of 50 μg/kg. The studied method is highly sensitive and reproducible, and can be used for the detection of sulfonamides in chicken.

Key words: sulfonamide compounds, ultra-high performance liquid chromatography (UHPLC), hollow fiber membrane phase microextraction

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