Journal of East China Normal University(Natural Sc ›› 2008, Vol. 2008 ›› Issue (2): 71-77.

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Cloning of zebrafish cyclin C cDNA and its developmental expression pattern(Chinese)

LIN Ai-fu1,2,JIA Fang-jun2,ZHENG Fu-jun2,3,WANG Qun1,YAN Yuan-chang2,4，LI Yi-ping2   

  1. 1. School of Life Science, East China Normal University, Shanghai200062, China; 2. Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences, Shanghai200031, China; 3. Bioinformation Center, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai200031, China; 4. Model Organism Division, E-Institutes of Shanghai Universities, Shanghai200031, China
  • Received:2007-01-26 Revised:2007-04-18 Online:2008-03-25 Published:2008-03-25
  • Contact: LI Yi-ping

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Abstract: Cyclin C binds the cyclin-dependent kinase 8 (cdk8) , which regulates transcription by phosphorylating RNA polymerase II and TFIIH. The effect of cyclin C on zygotic transcription activation during vertebrate embryonic development is poorly understood.This paper described the clone and developmental expression pattern of zebrafish cyclin C cDNA. It was highly conserved, sharing 88% amino acid sequence identity with human cyclin C, thereby indicating an important role for the protein. Northern blotting and whole mount in situ hybridisation revealed expression of zebrafish cyclin C maternally, following the onset of zygotic transcription at the mid-blastula transition (MBT) and throughout embryonic development.

Key words: expression, zebrafish, embryogenesis, cyclin C, expression, zebrafish, embryogenesis

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