Journal of East China Normal University(Natural Sc ›› 2007, Vol. 2007 ›› Issue (2): 104-110.

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Primary Study of CREB4 Translocation Mechanism from Cytoplasm to Nucleus(Chinese)

LIU Yong-qiang, NI Xiao-hua, ZHOU Dan, WANG Xin, GUO Wei-wei   

  1. School of Life Science, East China Normal University, Shanghai 200062, China
  • Received:2006-01-06 Revised:2006-05-24 Online:2007-03-25 Published:2007-03-25
  • Contact: NI Xiao-hua

Abstract: cAMP response element-binding(CREB) proteins are a family of mammalian transcription activators that mediate cAMP and calcium-dependent gene expression through the cAMP response element (CRE). CREB4 is a novel member of the human CREB family. Yeast two-hybrid system revealed that karyopherinα2 may associate with CREB4215-279aa. Subcellular location of CREB4 and CREB41-279aa showed that the GFP-CREB4 fusion protein was localized in cytoplasm, whereas the fusion protein of GFP-CREB41-279aa was translocated in nucleus. Our experiments further showed that CREB4 and karyopherinα2 were co-located in cytoplasm,but CREB41-279aa and karyopherinα2 were co-located in nucleus. These results suggested that first C-terminal was cleaved and then CREB4was carried into nucleus by karyopherinα2 to activate gene transcription.

Key words: yeast two-hybrid, fluorescent co-location, karyopherinα2, CREB4, yeast two-hybrid, fluorescent co-location, karyopherinα2

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