Metabolic potential and environmental adaptation mechanisms of Nitrospira in tidal flat wetlands

doi:10.3969/j.issn.1000-5641.2023.03.012

Abstract

Abstract:

To understand the metabolic potential and environmental adaptation mechanisms of Nitrospira in tidal flat wetlands, 14 high-quality Nitrospira genomes were constructed from five tidal flat wetlands along the coast of China using metagenomic binning and assembly methods. Phylogenetic analysis showed that among these genomes, three belonged to Comammox (complete ammonia oxidizer), nine belonged to lineage II and IV of Nitrospira, and two belonged to lineage III, which has yet to be discovered; taken together, this data suggests that abundant and diverse Nitrospira are present in China’s tidal flat wetlands. Metabolic reconstruction revealed that these Comammox and Nitrospira contained cyanase, urease, and other enzymes involved in the degradation of nitrile compounds and amide compounds; hence, they may utilize the organic nitrogen as energy by coupling with ammonia-oxidizing microorganisms. In addition, Nitrospira possessed flexible strategies to resist environmental stresses such as viral attack and osmotic changes. These results provide insights on the diversity, ecological function, and environmental adaptation mechanisms of Nitrospira from tidal wetlands.

Key words: Nitrospira, phylogeny, metabolic potential, environmental adaptation, tidal flat wetland

CLC Number:

Q938.1

Guohao CHEN, Tieqiang MAO, Hongpo DONG, Yafei OU, Jiawei ZHANG. Metabolic potential and environmental adaptation mechanisms of Nitrospira in tidal flat wetlands[J]. Journal of East China Normal University(Natural Science), 2023, 2023(3): 118-131.

Figures/Tables 8

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样品	站位	位置	深度/cm	盐度/‰	温度/℃	pH 值	采样时间	来源
CM01s	CM01	31°30′N, 121°58′E	15~20	0.9	9.3	8.16	2018年11月	崇明东滩湿地 (上海市)
CM01m	CM01	31°30′N, 121°58′E	55~60	1.0	9.3	8.10	2018年11月	崇明东滩湿地 (上海市)
CM01b	CM01	31°30′N, 121°58′E	95~100	1.1	9.3	8.22	2018年11月	崇明东滩湿地 (上海市)
CM02s	CM02	31°30′N, 121°58′E	15~20	1.2	9.2	7.99	2018年11月	崇明东滩湿地 (上海市)
CM02m	CM02	31°30′N, 121°58′E	55~60	0.9	9.2	8.36	2018年11月	崇明东滩湿地 (上海市)
CM02b	CM02	31°30′N, 121°58′E	95~100	0.9	9.2	8.37	2018年11月	崇明东滩湿地 (上海市)
QMs	QM	18°13′N, 109°37′E	0~5	9.0	23.5	7.17	2020年11月	青梅港红树林自然保护区 (海南省, 三亚)
QMm	QM	18°13′N, 109°37′E	10~15	11.0	23.5	7.43	2020年11月	青梅港红树林自然保护区 (海南省, 三亚)
QMb	QM	18°13′N, 109°37′E	25-30	12.0	23.5	7.54	2020年11月	青梅港红树林自然保护区 (海南省, 三亚)
LH01	LH01	40°47′N, 121°56′E	0~5	27.0	14.3	8.15	2019年03月	辽河口盐沼湿地 (辽宁省)
LH02	LH02	40°51′N, 121°56′E	0~5	26.0	12.0	7.93	2019年03月	辽河口盐沼湿地 (辽宁省)
ZN01	ZN01	24°20′N, 117°45′E					2015年09月	紫泥红树林自然保护区 (福建省, 龙海) ^[33]
ZN02	ZN02	24°20′N, 117°45′E					2015年09月	紫泥红树林自然保护区 (福建省, 龙海) ^[33]
YD	YD	37°49′N, 119°07′E					2017年12月	黄河三角洲湿地 (山东省)

基因组名称	序列数	基因组大小/Mb	GC含量/%	编码基因数	完整度/%	污染度/%
QMm6	86	3.34	64.94	3169	93.18	2.27
QMm125	437	3.29	51.51	3459	93.53	0
QMm174	409	3.16	49.78	3228	76.53	0.91
QMb135	78	3.30	64.94	3087	96.82	2.27
QMb347	295	4.20	50.36	4065	95.85	0.30
ZN01_bin277	601	3.64	55.70	3993	95.40	2.16
ZN02_bin95	568	3.66	55.78	4023	97.37	4.99
ZN02_bin129	407	3.49	49.77	3557	92.85	2.12
CM01m262	247	4.30	55.30	4495	77.04	0.91
CM01s42	677	3.05	55.63	3529	71.65	0.81
CM02s178	624	3.69	56.05	4084	80.30	1.75
CM02m401	591	3.47	55.53	3875	86.30	0.91
YD_bin17	653	2.16	57.60	2620	72.60	0.36
LH01_bin68	697	3.33	47.95	3659	81.20	2.73

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