Temperature adaptability of dark carbon fixation in seawater fromthe Yangtze River Estuary

doi:10.3969/j.issn.1000-5641.2024.01.011

Abstract

Abstract:

To clarify the effects of global warming on dark carbon fixation (DCF) in eutrophic estuaries, the rates of total DCF and DCF driven by ammonia-oxidizing microorganisms (DCF_AOB) were studied under various water temperatures and nitrogen concentrations using ¹⁴C labeling (NaH¹⁴CO₃) and the allylthiourea (ATU) inhibitor method. The Yangtze River Estuary was used as a study area and sampling locations were set up in the estuary and offshore locations. The DCF rates in the Yangtze River Estuary ranged from 0.23 to 0.33 μmolC·L^–1·d^–1 and that DCF_AOB rates accounted for 4.13% to 43.61% of the DCF. Although DCF rates increase significantly under optimum temperatures, the increase was more obvious with changes in ambient temperature under low salinity. The optimum temperatures for DCF in areas of low and high salinity were found to be 30℃ and 25℃, respectively, with the addition of ammonia-nitrogen at these conditions significantly increasing the DCF rates. The results of this study reveal how dark carbon fixation in estuarine water can change when subjected to environmental temperature changes, thereby providing theoretical support and data references to aid in the comprehensive understanding and scientific assessment of carbon fixation and carbon sink flux in estuarine ecosystems.

Key words: dark carbon fixation, the Yangtze River Estuary, ammonia-oxidizing microorganisms, salinity

CLC Number:

X142

Jiaming CHEN, Shiming WANG, Rongrong YANG, Ziyan CHEN, Xia LIANG, Lijun HOU. Temperature adaptability of dark carbon fixation in seawater fromthe Yangtze River Estuary[J]. Journal of East China Normal University(Natural Science), 2024, 2024(1): 104-112.

Figures/Tables 5

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Table 1

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温度/℃	不同${\rm{NH}}_4^+ $添加处理组
温度/℃	空白组	5.0 μmol·L^–1	10.0 μmol·L^–1	15.0 μmol·L^–1
20
25
30
35
40

采样断面	水体温度/℃	盐度/‰	${\rm{NH}}_4^+ $浓度/(μmol·L^–1)	${\rm{NO}}_3^- $浓度/(μmol·L^–1)	${\rm{NO}}_2^- $浓度/(μmol·L^–1)	DIC浓度/(mmol·L^–1)
近岸	28.5 ± 0.2	1.1 ± 0.01**	1.06 ± 0.29	107 ± 0.55**	0.09 ± 0.02*	1.73 ± 0.01*
近海	28.3 ± 0.1	32.6 ± 2.9**	0.67 ± 0.01	3.55 ± 1.90**	0.25 ± 0.07*	2.08 ± 0.16*

生态过程	区域	样本	培养温度/℃	Q₁₀	参考文献
暗碳固定	长江口	水体	20、30	1.1 ~ 2.5	本研究
	德国中部森林	山毛榉土壤	4、14	1.81 ± 0.17	Akinyede等^[13]
	德国中部森林	云杉土壤	4、14	2.07 ± 0.34	Akinyede等^[13]
氨氧化	埃姆斯多拉德河口	AOB	10、20	3.3	Helder等^[30]
氨氧化	普吉特湾河口	AOA	10、20	2.5 ~ 2.9	Qin等^[15]
呼吸	温带针叶林	土壤	4	2.4	Hicks等^[29]

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