Journal of East China Normal University(Natural Sc ›› 2005, Vol. 2005 ›› Issue (4): 73-77.

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Comparative Investigation of Cytoskeleton Proteins between KAx-3 and AK127 Cells during Dictyostelium Development(Chinese)

HOU Lian-sheng, FU Zou-min   

  1. School of Life Science, East China Normal University, Shanghai 200062, China
  • Received:2004-02-18 Revised:2004-03-24 Online:2005-11-25 Published:2005-11-25
  • Contact: HOU Lian-sheng

Abstract: Cell of Dictyostelium discoideum are trigged to undergo development when they are depleted their food source. Cell cytoskeleton proteins play an important role for completing the development of Dictyostelium discoideum. To study the change of cytoskeleton proteins during Dictyostelium discoideum development, we used the wild-type cells KAx-3 and mutant cells AK127; cytoskeleton ‘ghost’ have been prepared by biochemical extraction with TrotonX-100, cytoskeleton proteins were separated using SDS-PAGE and scan by BIO-PROFILE of VILBER LOURMAT. The datum showed that the 103.4kD、49.6kD、44.2kD、30.8kD、19.8kD and 13.5kD were shared by KAx-3 and AK127 cells; in that 44.2kD and 30.8kD were the most stable. They may take some support for cell development with other cytoskeleton proteins. The most different existed in some proteins, for example 87.2kD, 68.2kD and 40.7kD, those proteins can not be expressed in the mutant cells at the same developmental stage of wild type cells. This result indicates those proteins are required for Dictyostelium cells development. It is noteworthy that mutant cell showed the characteristic 21.1kD proteins after 12h development. These obvious difference related to the process of development, in other hand, also related to the mutant cell lacking gp150 protein.

Key words: development, cytoskeleton proteins, Dictyostelium discoideum, development, cytoskeleton proteins

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